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Volume 12
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10.3390/antibiotics12010041
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Article

Isolation, Identification and Genetic Characterization of Antibiotic Resistant Escherichia coli from Frozen Chicken Meat Obtained from Supermarkets at Dhaka City in Bangladesh

by
Mridha. Md. Kamal Hossain
1,
Md. Sharifull Islam
1,2,
Md. Salah Uddin
1,3,
A. T. M. Mijanur Rahman
3,
Asad Ud-Daula
3,
Md. Ariful Islam
1,3,
Rubaya Rubaya
1,
Anjuman Ara Bhuiya
1,
Md. Abdul Alim
1,
Nusrat Jahan
1,
Jinquan Li
4,* and
Jahangir Alam
1,*
1
Animal Biotechnology Division, National Institute of Biotechnology, Savar 1349, Bangladesh
2
Center for Cancer Immunology, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
3
Department of Applied Nutrition and Food Technology, Islamic University, Kushtia 7003, Bangladesh
4
State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
*
Authors to whom correspondence should be addressed.
Antibiotics 2023, 12(1), 41; https://doi.org/10.3390/antibiotics12010041
Submission received: 29 November 2022 / Revised: 13 December 2022 / Accepted: 22 December 2022 / Published: 27 December 2022
(This article belongs to the Special Issue Insights into Antibiotics in Human, Animal, and Agriculture: Resistance, Determinant, and Treatment)
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Abstract

Antimicrobials have been used to improve animal welfare, food security, and food safety that promote the emergence, selection, and dissemination of antimicrobial-resistant (AMR) bacteria. In this study, 50 E. coli were isolated from frozen chicken meat samples in Dhaka city. Antibiotic sensitivity patterns were assessed through the disk diffusion method and finally screened for the presence of antimicrobial resistance genes (ARG) using the polymerase chain reaction (PCR). Among the 160 samples, the prevalence of E. coli was observed in fifty samples (31.25%). All of these isolates were found resistant to at least one antimicrobial agent, and 52.0% of the isolates were resistant against 4–7 different antimicrobials. High resistance was shown to tetracycline (66.0%), followed by resistance to erythromycin (42.0%), ampicillin and streptomycin (38.0%), and sulfonamide (28.0%). In addition, the most prevalent ARGs were tet(A) (66.0%), ereA (64.0%), tet(B) (60.0%), aadA1 and sulI (56.0%), blaCITM (48.0%) and blaSHV (40.0%). About 90.0% of isolates were multidrug resistant. This study reveals for the first time the current situation of E. coli AMR in broilers, which is helpful for the clinical control of disease as well as for the development of policies and guidelines to reduce AMR in broilers production in Bangladesh.
Keywords: frozen chicken meat; E. coli; Antimicrobials; Antimicrobial resistance genes; Dhaka city frozen chicken meat; E. coli; Antimicrobials; Antimicrobial resistance genes; Dhaka city

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MDPI and ACS Style

Hossain, M.M.K.; Islam, M.S.; Uddin, M.S.; Rahman, A.T.M.M.; Ud-Daula, A.; Islam, M.A.; Rubaya, R.; Bhuiya, A.A.; Alim, M.A.; Jahan, N.; et al. Isolation, Identification and Genetic Characterization of Antibiotic Resistant Escherichia coli from Frozen Chicken Meat Obtained from Supermarkets at Dhaka City in Bangladesh. Antibiotics 2023, 12, 41. https://doi.org/10.3390/antibiotics12010041

AMA Style

Hossain MMK, Islam MS, Uddin MS, Rahman ATMM, Ud-Daula A, Islam MA, Rubaya R, Bhuiya AA, Alim MA, Jahan N, et al. Isolation, Identification and Genetic Characterization of Antibiotic Resistant Escherichia coli from Frozen Chicken Meat Obtained from Supermarkets at Dhaka City in Bangladesh. Antibiotics. 2023; 12(1):41. https://doi.org/10.3390/antibiotics12010041

Chicago/Turabian Style

Hossain, Mridha. Md. Kamal, Md. Sharifull Islam, Md. Salah Uddin, A. T. M. Mijanur Rahman, Asad Ud-Daula, Md. Ariful Islam, Rubaya Rubaya, Anjuman Ara Bhuiya, Md. Abdul Alim, Nusrat Jahan, and et al. 2023. "Isolation, Identification and Genetic Characterization of Antibiotic Resistant Escherichia coli from Frozen Chicken Meat Obtained from Supermarkets at Dhaka City in Bangladesh" Antibiotics 12, no. 1: 41. https://doi.org/10.3390/antibiotics12010041

APA Style

Hossain, M. M. K., Islam, M. S., Uddin, M. S., Rahman, A. T. M. M., Ud-Daula, A., Islam, M. A., Rubaya, R., Bhuiya, A. A., Alim, M. A., Jahan, N., Li, J., & Alam, J. (2023). Isolation, Identification and Genetic Characterization of Antibiotic Resistant Escherichia coli from Frozen Chicken Meat Obtained from Supermarkets at Dhaka City in Bangladesh. Antibiotics, 12(1), 41. https://doi.org/10.3390/antibiotics12010041

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