Evaluation of the Efficiency of Random and Diblock Methacrylate-Based Amphiphilic Cationic Polymers against Major Bacterial Pathogens Associated with Cystic Fibrosis
Abstract
:1. Introduction
2. Results
2.1. Antimicrobial Activity of the Copolymers
2.2. Innocuity of the Copolymers
2.3. Determination of Time-Kill Kinetics
2.4. Mechanism of Action
2.5. Insertion in Lipid Monolayers
2.6. Resistance Induction Assay
2.7. Anti-Biofilm Activity
3. Discussion
4. Materials and Methods
4.1. Synthesis and Characterization of the Cationic Amphiphilic Polymers
4.2. Bacterial Strains and Growth Conditions
4.3. Minimal Inhibitory Concentration (MIC) Determination
4.4. Evaluation of the Cytotoxic Effect of Polymers Using Human Cells
4.5. Time–Kill Assays
4.6. Bacterial Membrane Permeabilization Assay
4.7. Induction of Resistance Assay
4.8. Lipid Insertion Assay
4.9. Antibiofilm Formation Assay
4.10. Antibiofilm Disruption Assay
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Copolymer Name | Mn (g/mol) (SEC/DMF) | FDMAEMA |
---|---|---|
Random2800 | 2800 | 0.67 |
Random3300 | 3300 | 0.63 |
Random9300 | 9300 | 0.65 |
Random10200 | 10,200 | 0.64 |
Random14600 | 14,600 | 0.65 |
Random15000 | 15,000 | 0.56 |
Random18500 | 18,500 | 0.68 |
Random23900 | 23,900 | 0.61 |
Diblock6800 | 6800 | 0.65 |
Diblock9500 | 9500 | 0.62 |
Diblock9900 | 9900 | 0.72 |
Diblock14700 | 14,700 | 0.78 |
Diblock17900 | 17,900 | 0.62 |
Diblock18000 | 18,000 | 0.8 |
Diblock18400 | 18,400 | 0.64 |
Diblock18800 | 18,800 | 0.57 |
Diblock19200 | 19,200 | 0.74 |
Diblock20500 | 20,500 | 0.64 |
Diblock21900 | 21,900 | 0.58 |
Copolymer Name | M. abscessus S | P. aeruginosa | S. aureus |
---|---|---|---|
Random2800 | 6.25 | 200 | 200 |
Random3300 | 25 | 400 | 200 |
Random9300 | 25 | 25 | 50 |
Random10200 | 12.5 | 25 | 50 |
Random14600 | 25 | 25 | 12.5 |
Random15000 | 25 | 25 | 25 |
Random18500 | 25 | 12.5 | 12.5 |
Random23900 | 25 | 12.5 | 12.5 |
Diblock6800 | 25 | 100 | 12.5 |
Diblock9500 | 50 | 50 | 12.5 |
Diblock9900 | 50 | 50 | 12.5 |
Diblock14700 | 50 | 50 | 12.5 |
Diblock17900 | 100 | 50 | 12.5 |
Diblock18000 | 50 | 50 | 12.5 |
Diblock18400 | 100 | 50 | 25 |
Diblock18800 | 100 | 50 | 12.5 |
Diblock19200 | 50 | 50 | 12.5 |
Diblock20500 | 50 | 100 | 12.5 |
Diblock21900 | 100 | 50 | 12.5 |
Copolymer Name | IC50 BEAS-2B | TI M. abscessus S | TI P. aeruginosa | TI S. aureus |
---|---|---|---|---|
Random2800 | >400 | >64 | >2 | >2 |
Random3300 | >400 | >16 | >1 | >2 |
Random9300 | 195.1 | 7.8 | 7.8 | 3.9 |
Random10200 | 231.1 | 18.5 | 9.2 | 4.6 |
Random14600 | 133.7 | 5.3 | 5.3 | 10.7 |
Random15000 | 148.3 | 5.9 | 5.9 | 5.9 |
Random18500 | 124.7 | 5.0 | 10.0 | 10.0 |
Random23900 | 204.2 | 8.2 | 16.3 | 16.3 |
Diblock6800 | 228.4 | 9.1 | 2.3 | 18.3 |
Diblock9500 | 250.6 | 5.0 | 5.0 | 20.0 |
Diblock9900 | 244.5 | 4.9 | 4.9 | 19.6 |
Diblock14700 | 211.5 | 4.2 | 4.2 | 16.9 |
Diblock17900 | 172.8 | 1.7 | 3.4 | 13.8 |
Diblock18000 | 219.2 | 4.4 | 4.4 | 17.5 |
Diblock18400 | 242.1 | 2.4 | 4.8 | 9.7 |
Diblock18800 | 232.1 | 2.3 | 4.6 | 18.6 |
Diblock19200 | 161.8 | 3.2 | 3.2 | 12.9 |
Diblock20500 | 222.7 | 4.4 | 2.2 | 17.8 |
Diblock21900 | 239 | 2.4 | 4.8 | 19.1 |
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Casanova, M.; Olleik, H.; Hdiouech, S.; Roblin, C.; Cavalier, J.-F.; Point, V.; Jeannot, K.; Caron, B.; Perrier, J.; Charriau, S.; et al. Evaluation of the Efficiency of Random and Diblock Methacrylate-Based Amphiphilic Cationic Polymers against Major Bacterial Pathogens Associated with Cystic Fibrosis. Antibiotics 2023, 12, 120. https://doi.org/10.3390/antibiotics12010120
Casanova M, Olleik H, Hdiouech S, Roblin C, Cavalier J-F, Point V, Jeannot K, Caron B, Perrier J, Charriau S, et al. Evaluation of the Efficiency of Random and Diblock Methacrylate-Based Amphiphilic Cationic Polymers against Major Bacterial Pathogens Associated with Cystic Fibrosis. Antibiotics. 2023; 12(1):120. https://doi.org/10.3390/antibiotics12010120
Chicago/Turabian StyleCasanova, Magali, Hamza Olleik, Slim Hdiouech, Clarisse Roblin, Jean-François Cavalier, Vanessa Point, Katy Jeannot, Baptiste Caron, Josette Perrier, Siméon Charriau, and et al. 2023. "Evaluation of the Efficiency of Random and Diblock Methacrylate-Based Amphiphilic Cationic Polymers against Major Bacterial Pathogens Associated with Cystic Fibrosis" Antibiotics 12, no. 1: 120. https://doi.org/10.3390/antibiotics12010120