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Article

Molecular Analysis of Streptomycin Resistance Genes in Clinical Strains of Mycobacterium tuberculosis and Biocomputational Analysis of the MtGidB L101F Variant

1
Biotechnology and Biosciences Research Group, Faculty of Chemical Sciences and Engineering, Autonomous University of Baja California, Tijuana 22390, Mexico
2
Clinical Diagnostic Laboratory, General Hospital of Tijuana, Tijuana 22010, Mexico
3
Department of Biomedical Innovation, Center for Scientific Research and Higher Education at Ensenada, Ensenada 22860, Mexico
*
Authors to whom correspondence should be addressed.
Academic Editor: Danila V. Zimenkov
Antibiotics 2021, 10(7), 807; https://doi.org/10.3390/antibiotics10070807
Received: 1 June 2021 / Revised: 29 June 2021 / Accepted: 30 June 2021 / Published: 2 July 2021
Globally, tuberculosis (TB) remains a prevalent threat to public health. In 2019, TB affected 10 million people and caused 1.4 million deaths. The major challenge for controlling this infectious disease is the emergence and spread of drug-resistant Mycobacterium tuberculosis, the causative agent of TB. The antibiotic streptomycin is not a current first-line anti-TB drug. However, WHO recommends its use in patients infected with a streptomycin-sensitive strain. Several mutations in the M. tuberculosisrpsL, rrs and gidB genes have proved association with streptomycin resistance. In this study, we performed a molecular analysis of these genes in clinical isolates to determine the prevalence of known or novel mutations. Here, we describe the genetic analysis outcome. Furthermore, a biocomputational analysis of the MtGidB L101F variant, the product of a novel mutation detected in gidB during molecular analysis, is also reported as a theoretical approach to study the apparent genotype-phenotype association. View Full-Text
Keywords: Mycobacterium tuberculosis; streptomycin resistance; molecular analysis; biocomputational analysis; clinical isolates Mycobacterium tuberculosis; streptomycin resistance; molecular analysis; biocomputational analysis; clinical isolates
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MDPI and ACS Style

Rodríguez-García, Á.; Mares-Alejandre, R.E.; Muñoz-Muñoz, P.L.A.; Ruvalcaba-Ruiz, S.; González-Sánchez, R.A.; Bernáldez-Sarabia, J.; Meléndez-López, S.G.; Licea-Navarro, A.F.; Ramos-Ibarra, M.A. Molecular Analysis of Streptomycin Resistance Genes in Clinical Strains of Mycobacterium tuberculosis and Biocomputational Analysis of the MtGidB L101F Variant. Antibiotics 2021, 10, 807. https://doi.org/10.3390/antibiotics10070807

AMA Style

Rodríguez-García Á, Mares-Alejandre RE, Muñoz-Muñoz PLA, Ruvalcaba-Ruiz S, González-Sánchez RA, Bernáldez-Sarabia J, Meléndez-López SG, Licea-Navarro AF, Ramos-Ibarra MA. Molecular Analysis of Streptomycin Resistance Genes in Clinical Strains of Mycobacterium tuberculosis and Biocomputational Analysis of the MtGidB L101F Variant. Antibiotics. 2021; 10(7):807. https://doi.org/10.3390/antibiotics10070807

Chicago/Turabian Style

Rodríguez-García, Álvaro, Rosa E. Mares-Alejandre, Patricia L. A. Muñoz-Muñoz, Samuel Ruvalcaba-Ruiz, Ricardo A. González-Sánchez, Johanna Bernáldez-Sarabia, Samuel G. Meléndez-López, Alexei F. Licea-Navarro, and Marco A. Ramos-Ibarra. 2021. "Molecular Analysis of Streptomycin Resistance Genes in Clinical Strains of Mycobacterium tuberculosis and Biocomputational Analysis of the MtGidB L101F Variant" Antibiotics 10, no. 7: 807. https://doi.org/10.3390/antibiotics10070807

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