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Open AccessArticle

Transmission of Similar Mcr-1 Carrying Plasmids among Different Escherichia coli Lineages Isolated from Livestock and the Farmer

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Servei Veterinari de Genètica Molecular (SVGM), Universitat Autònoma de Barcelona, 08193 Barcelona, Spain
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Vetgenomics, Edifici EUREKA, Parc de Recerca de la UAB, Campus UAB, 08193 Barcelona, Spain
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IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus UAB, Universitat Autònoma de Barcelona, 08193 Barcelona, Spain
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OIE Collaborating Centre for the Research and Control of Emerging and Re-emerging Swine Diseases in Europe (IRTA-CReSA), 08193 Barcelona, Spain
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Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, 28040 Madrid, Spain
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Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
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Area de Programas Sanitarios y Zoonosis, S.G. de Sanidad e Higiene Animal y Trazabilidad, Ministerio de Agricultura, Pesca y Alimentación, 28014 Madrid, Spain
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Departament d’Agricultura, Ramaderia, Pesca i Alimentació, Servei d’Alimentació Animal i Seguretat de la Producció Ramadera, 08007 Barcelona, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Kazuki Harada
Antibiotics 2021, 10(3), 313; https://doi.org/10.3390/antibiotics10030313
Received: 16 February 2021 / Revised: 15 March 2021 / Accepted: 16 March 2021 / Published: 17 March 2021
(This article belongs to the Special Issue Multidrug-Resistant Bacteria in Animals)
Colistin use has mostly been stopped in human medicine, due to its toxicity. However, nowadays, it still is used as a last-resort antibiotic to treat hospital infections caused by multi-drug resistant Enterobacteriaceae. On the contrary, colistin has been used in veterinary medicine until recently. In this study, 210 fecal samples from pigs (n = 57), calves (n = 152), and the farmer (n = 1) were collected from a farm where E. coli harboring mcr-1–mcr-3 was previously detected. Samples were plated, and mcr-genes presence was confirmed by multiplex-PCR. Hybrid sequencing which determined the presence and location of mcr-1, other antibiotic resistance genes, and virulence factors. Eighteen colistin resistant isolates (13 from calves, four from pigs, and one from the farmer) contained mcr-1 associated with plasmids (IncX4, IncI2, and IncHI2), except for two that yielded mcr-1 in the chromosome. Similar plasmids were distributed in different E. coli lineages. Transmission of mcr-1 to the farmer most likely occurred by horizontal gene transfer from E. coli of calf origin, since plasmids were highly similar (99% coverage, 99.97% identity). Moreover, 33 virulence factors, including stx2 for Shiga toxin E. coli (STEC) were detected, highlighting the role of livestock as a reservoir of pathotypes with zoonotic potential. View Full-Text
Keywords: Escherichia coli; colistin; mcr; plasmids; MinION nanopore; hybrid sequencing; livestock Escherichia coli; colistin; mcr; plasmids; MinION nanopore; hybrid sequencing; livestock
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MDPI and ACS Style

Viñes, J.; Cuscó, A.; Napp, S.; Alvarez, J.; Saez-Llorente, J.L.; Rosàs-Rodoreda, M.; Francino, O.; Migura-Garcia, L. Transmission of Similar Mcr-1 Carrying Plasmids among Different Escherichia coli Lineages Isolated from Livestock and the Farmer. Antibiotics 2021, 10, 313. https://doi.org/10.3390/antibiotics10030313

AMA Style

Viñes J, Cuscó A, Napp S, Alvarez J, Saez-Llorente JL, Rosàs-Rodoreda M, Francino O, Migura-Garcia L. Transmission of Similar Mcr-1 Carrying Plasmids among Different Escherichia coli Lineages Isolated from Livestock and the Farmer. Antibiotics. 2021; 10(3):313. https://doi.org/10.3390/antibiotics10030313

Chicago/Turabian Style

Viñes, Joaquim; Cuscó, Anna; Napp, Sebastian; Alvarez, Julio; Saez-Llorente, Jose L.; Rosàs-Rodoreda, Montserrat; Francino, Olga; Migura-Garcia, Lourdes. 2021. "Transmission of Similar Mcr-1 Carrying Plasmids among Different Escherichia coli Lineages Isolated from Livestock and the Farmer" Antibiotics 10, no. 3: 313. https://doi.org/10.3390/antibiotics10030313

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