Next Article in Journal
Fast Active Merging of Microdroplets in Microfluidic Chambers Driven by Photo-Isomerisation of Azobenzene Based Surfactants
Previous Article in Journal
What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications
Open AccessArticle

A Sustainable Biomineralization Approach for the Synthesis of Highly Fluorescent Ultra-Small Pt Nanoclusters

1
Chemical and Environment Engineering, School of Engineering, RMIT University, Melbourne, VIC 3000, Australia
2
Australian Centre for Neutron Scattering (ACNS), Australian Nuclear Science and Technology Organisation (ANSTO), Lucas Heights, NSW 2234, Australia
3
CSIRO Agriculture, Level 6, Queensland Bioscience Precinct, St Lucia, QLD 4067, Australia
4
CSIRO Manufacturing, Bayview Ave, Clayton, VIC 3168, Australia
*
Authors to whom correspondence should be addressed.
Biosensors 2019, 9(4), 128; https://doi.org/10.3390/bios9040128
Received: 14 August 2019 / Revised: 16 October 2019 / Accepted: 21 October 2019 / Published: 29 October 2019
Herein we report the first example of a facile biomineralization process to produce ultra-small-sized highly fluorescent aqueous dispersions of platinum noble metal quantum clusters (Pt-NMQCs) using a multi-stimulus responsive, biomimetic intrinsically disordered protein (IDP), Rec1-resilin. We demonstrate that Rec1-resilin acts concurrently as the host, reducing agent, and stabilizer of the blue-green fluorescent Pt-NMQCs once they are being formed. The photophysical properties, quantum yield, and fluorescence lifetime measurements of the synthesized Pt-NMQCs were examined using UV-Vis and fluorescence spectroscopy. The oxidation state of the Pt-NMQCs was quantitatively analyzed using X-ray photoelectron spectroscopy. Both a small angle X-ray scattering technique and a modeling approach have been attempted to present a detailed understanding of the structure and conformational dynamics of Rec1-resilin as an IDP during the formation of the Pt-NMQCs. It has been demonstrated that the green fluorescent Pt-NMQCs exhibit a high quantum yield of ~7.0% and a lifetime of ~9.5 ns in aqueous media. The change in photoluminescence properties due to the inter-dot interactions between proximal dots and aggregation of the Pt-NMQCs by evaporation was also measured spectroscopically and discussed. View Full-Text
Keywords: quantum dot; noble metal clusters; fluorescent platinum nanoclusters; biosensors; biomineralization; intrinsically disordered protein; protein polymer; small angle X-ray scattering quantum dot; noble metal clusters; fluorescent platinum nanoclusters; biosensors; biomineralization; intrinsically disordered protein; protein polymer; small angle X-ray scattering
Show Figures

Graphical abstract

MDPI and ACS Style

Balu, R.; Knott, R.; Elvin, C.M.; Hill, A.J.; R. Choudhury, N.; Dutta, N.K. A Sustainable Biomineralization Approach for the Synthesis of Highly Fluorescent Ultra-Small Pt Nanoclusters. Biosensors 2019, 9, 128.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop