Next Article in Journal
Fast Active Merging of Microdroplets in Microfluidic Chambers Driven by Photo-Isomerisation of Azobenzene Based Surfactants
Previous Article in Journal
What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications
Open AccessArticle

A Sustainable Biomineralization Approach for the Synthesis of Highly Fluorescent Ultra-Small Pt Nanoclusters

1
Chemical and Environment Engineering, School of Engineering, RMIT University, Melbourne, VIC 3000, Australia
2
Australian Centre for Neutron Scattering (ACNS), Australian Nuclear Science and Technology Organisation (ANSTO), Lucas Heights, NSW 2234, Australia
3
CSIRO Agriculture, Level 6, Queensland Bioscience Precinct, St Lucia, QLD 4067, Australia
4
CSIRO Manufacturing, Bayview Ave, Clayton, VIC 3168, Australia
*
Authors to whom correspondence should be addressed.
Biosensors 2019, 9(4), 128; https://doi.org/10.3390/bios9040128
Received: 14 August 2019 / Revised: 16 October 2019 / Accepted: 21 October 2019 / Published: 29 October 2019
Herein we report the first example of a facile biomineralization process to produce ultra-small-sized highly fluorescent aqueous dispersions of platinum noble metal quantum clusters (Pt-NMQCs) using a multi-stimulus responsive, biomimetic intrinsically disordered protein (IDP), Rec1-resilin. We demonstrate that Rec1-resilin acts concurrently as the host, reducing agent, and stabilizer of the blue-green fluorescent Pt-NMQCs once they are being formed. The photophysical properties, quantum yield, and fluorescence lifetime measurements of the synthesized Pt-NMQCs were examined using UV-Vis and fluorescence spectroscopy. The oxidation state of the Pt-NMQCs was quantitatively analyzed using X-ray photoelectron spectroscopy. Both a small angle X-ray scattering technique and a modeling approach have been attempted to present a detailed understanding of the structure and conformational dynamics of Rec1-resilin as an IDP during the formation of the Pt-NMQCs. It has been demonstrated that the green fluorescent Pt-NMQCs exhibit a high quantum yield of ~7.0% and a lifetime of ~9.5 ns in aqueous media. The change in photoluminescence properties due to the inter-dot interactions between proximal dots and aggregation of the Pt-NMQCs by evaporation was also measured spectroscopically and discussed. View Full-Text
Keywords: quantum dot; noble metal clusters; fluorescent platinum nanoclusters; biosensors; biomineralization; intrinsically disordered protein; protein polymer; small angle X-ray scattering quantum dot; noble metal clusters; fluorescent platinum nanoclusters; biosensors; biomineralization; intrinsically disordered protein; protein polymer; small angle X-ray scattering
Show Figures

Graphical abstract

MDPI and ACS Style

Balu, R.; Knott, R.; Elvin, C.M.; Hill, A.J.; R. Choudhury, N.; Dutta, N.K. A Sustainable Biomineralization Approach for the Synthesis of Highly Fluorescent Ultra-Small Pt Nanoclusters. Biosensors 2019, 9, 128. https://doi.org/10.3390/bios9040128

AMA Style

Balu R, Knott R, Elvin CM, Hill AJ, R. Choudhury N, Dutta NK. A Sustainable Biomineralization Approach for the Synthesis of Highly Fluorescent Ultra-Small Pt Nanoclusters. Biosensors. 2019; 9(4):128. https://doi.org/10.3390/bios9040128

Chicago/Turabian Style

Balu, Rajkamal; Knott, Robert; Elvin, Christopher M.; Hill, Anita J.; R. Choudhury, Namita; Dutta, Naba K. 2019. "A Sustainable Biomineralization Approach for the Synthesis of Highly Fluorescent Ultra-Small Pt Nanoclusters" Biosensors 9, no. 4: 128. https://doi.org/10.3390/bios9040128

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop