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In Vivo Biosensing Using Resonance Energy Transfer
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FRET Microscopy in Yeast

by Michal Skruzny 1,2,*, Emma Pohl 1,2 and Marc Abella 1,2
1
Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany
2
LOEWE Center for Synthetic Microbiology (SYNMIKRO), 35043 Marburg, Germany
*
Author to whom correspondence should be addressed.
Biosensors 2019, 9(4), 122; https://doi.org/10.3390/bios9040122
Received: 30 June 2019 / Revised: 19 September 2019 / Accepted: 30 September 2019 / Published: 11 October 2019
(This article belongs to the Special Issue FRET-Based Biosensors)
Förster resonance energy transfer (FRET) microscopy is a powerful fluorescence microscopy method to study the nanoscale organization of multiprotein assemblies in vivo. Moreover, many biochemical and biophysical processes can be followed by employing sophisticated FRET biosensors directly in living cells. Here, we summarize existing FRET experiments and biosensors applied in yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, two important models of fundamental biomedical research and efficient platforms for analyses of bioactive molecules. We aim to provide a practical guide on suitable FRET techniques, fluorescent proteins, and experimental setups available for successful FRET experiments in yeasts. View Full-Text
Keywords: budding yeast; fission yeast; acceptor photobleaching; sensitized emission; ratiometric FRET; FLIM; GFP budding yeast; fission yeast; acceptor photobleaching; sensitized emission; ratiometric FRET; FLIM; GFP
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MDPI and ACS Style

Skruzny, M.; Pohl, E.; Abella, M. FRET Microscopy in Yeast. Biosensors 2019, 9, 122.

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