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Biosensors 2018, 8(4), 131; https://doi.org/10.3390/bios8040131

Label-Free SERS Discrimination and In Situ Analysis of Life Cycle in Escherichia coli and Staphylococcus epidermidis

1
Department of Applied Science and Technology, Politecnico di Torino, C.so Duca degli Abruzzi 24, 10129 Turin, Italy
2
Ceramic Physics Laboratory, Kyoto Institute of Technology, Kyoto 606-8585, Japan
3
Department of Immunology, Kyoto Prefectural University of Medicine, Kyoto 602-0841, Japan
4
Department of Dental Medicine, Kyoto Prefectural University of Medicine, Kyoto 602-0841, Japan
5
Department of Orthopedic Surgery, Tokyo Medical University, Tokyo 160-8402, Japan
6
The Center for Advanced Medical Engineering and Informatics, Osaka University, Osaka 565-0871, Japan
*
Authors to whom correspondence should be addressed.
Received: 13 November 2018 / Revised: 11 December 2018 / Accepted: 13 December 2018 / Published: 15 December 2018
(This article belongs to the Special Issue Applications of Raman Techniques in Biosensing)
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Abstract

Surface enhanced Raman spectroscopy (SERS) has been proven suitable for identifying and characterizing different bacterial species, and to fully understand the chemically driven metabolic variations that occur during their evolution. In this study, SERS was exploited to identify the cellular composition of Gram-positive and Gram-negative bacteria by using mesoporous silicon-based substrates decorated with silver nanoparticles. The main differences between the investigated bacterial strains reside in the structure of the cell walls and plasmatic membranes, as well as their biofilm matrix, as clearly noticed in the corresponding SERS spectrum. A complete characterization of the spectra was provided in order to understand the contribution of each vibrational signal collected from the bacterial culture at different times, allowing the analysis of the bacterial populations after 12, 24, and 48 h. The results show clear features in terms of vibrational bands in line with the bacterial growth curve, including an increasing intensity of the signals during the first 24 h and their subsequent decrease in the late stationary phase after 48 h of culture. The evolution of the bacterial culture was also confirmed by fluorescence microscope images. View Full-Text
Keywords: SERS; metal-dielectric nanostructures; E. coli; S. epidermidis; biofilm SERS; metal-dielectric nanostructures; E. coli; S. epidermidis; biofilm
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Paccotti, N.; Boschetto, F.; Horiguchi, S.; Marin, E.; Chiadò, A.; Novara, C.; Geobaldo, F.; Giorgis, F.; Pezzotti, G. Label-Free SERS Discrimination and In Situ Analysis of Life Cycle in Escherichia coli and Staphylococcus epidermidis. Biosensors 2018, 8, 131.

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