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Biosensors 2018, 8(4), 123;

Biomolecular Component Analysis of Phospholipids Composition in Live HeLa Cells

Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
Author to whom correspondence should be addressed.
Received: 22 October 2018 / Revised: 30 November 2018 / Accepted: 3 December 2018 / Published: 5 December 2018
(This article belongs to the Special Issue Applications of Raman Techniques in Biosensing)
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The alteration of the phospholipid composition within the cell, in particular the ratio between saturated and unsaturated fatty acids, can serve as an important biomarker to prognosis of the disease progression (e.g., fatty-liver disease, prostate cancer, or neurodegenerative disorders). Major techniques for lipid analysis in biological samples require a lipid extraction procedure that is not compatible with live cell studies. To address this challenge, we apply microRaman-Biomolecular Component Analysis (BCA) for comparative analysis of phospholipid composition and sensing the saturation degree of fatty acid lipid chain in live HeLa cells and lipids extracted from HeLa cells. After processing raw Raman data, acquired in lipid droplets (LDs) free cytoplasmic area, LDs and extracted lipids with BCA, the lipid component was isolated. Despite the similarity in general profiles of processed Raman spectra acquired in live cells and extracted lipids, some clear differences that reflect diversity in their phospholipids composition were revealed. Furthermore, using the direct relation between the number of double bonds in the fatty acid chain and the intensity ratio of the corresponding Raman bands, the saturation degree of fatty acids was estimated. View Full-Text
Keywords: BCA; Raman microspectrometry; phospholipids; fatty acids; HeLa cells BCA; Raman microspectrometry; phospholipids; fatty acids; HeLa cells

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Levchenko, S.M.; Qu, J. Biomolecular Component Analysis of Phospholipids Composition in Live HeLa Cells. Biosensors 2018, 8, 123.

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