Next Article in Journal
Sensors Based on Metal Nanoclusters Stabilized on Designed Proteins
Previous Article in Journal
Metal Oxide Nanoparticle Based Electrochemical Sensor for Total Antioxidant Capacity (TAC) Detection in Wine Samples
Article Menu

Export Article

Open AccessArticle
Biosensors 2018, 8(4), 109; https://doi.org/10.3390/bios8040109

Electrochemical Determination of β-Lactoglobulin Employing a Polystyrene Bead-Modified Carbon Nanotube Ink

1
U.T. Nanomateriales, INTI-Procesos Superficiales, Instituto Nacional de Tecnología Industrial, Av. Gral. Paz 5445, San Martín B1650WAB, Argentina
2
Centro de Micro y Nanoelectrónica, Instituto Nacional de Tecnología Industrial, Av. Gral. Paz 5445, San Martín B1650WAB, Argentina
3
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires C1033AAJ, Argentina
*
Author to whom correspondence should be addressed.
Received: 27 September 2018 / Revised: 6 November 2018 / Accepted: 8 November 2018 / Published: 15 November 2018
(This article belongs to the Special Issue Electrochemical Immunosensor)
Full-Text   |   PDF [2469 KB, uploaded 15 November 2018]   |  

Abstract

In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of β-lactoglobulin. Detection of this protein from cow’s milk was performed by a sandwich immunoassay onto printed carbon nanotube electrodes. The electrodes were printed using a carbon nanotube ink modified with polystyrene beads containing a high amount of carboxylic groups for protein immobilization. This strategy showed enhanced sensing performance compared to the use of oxidative treatments for the functionalization of electrodes. These electrodes showed an excellent electrochemical behavior, and proteins could be immobilized on their surface via the carbodiimide reaction. These antibody-immobilized carbon nanotube electrodes allowed for the detection of β-lactoglobulin in sub-ppm concentrations. View Full-Text
Keywords: electrochemical biosensor; carbon nanotubes; food allergen; β-lactoglobulin electrochemical biosensor; carbon nanotubes; food allergen; β-lactoglobulin
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Molinari, J.; Florez, L.; Medrano, A.; Monsalve, L.; Ybarra, G. Electrochemical Determination of β-Lactoglobulin Employing a Polystyrene Bead-Modified Carbon Nanotube Ink. Biosensors 2018, 8, 109.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top