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Open AccessArticle

Electrochemical Determination of β-Lactoglobulin Employing a Polystyrene Bead-Modified Carbon Nanotube Ink

1
U.T. Nanomateriales, INTI-Procesos Superficiales, Instituto Nacional de Tecnología Industrial, Av. Gral. Paz 5445, San Martín B1650WAB, Argentina
2
Centro de Micro y Nanoelectrónica, Instituto Nacional de Tecnología Industrial, Av. Gral. Paz 5445, San Martín B1650WAB, Argentina
3
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires C1033AAJ, Argentina
*
Author to whom correspondence should be addressed.
Biosensors 2018, 8(4), 109; https://doi.org/10.3390/bios8040109
Received: 27 September 2018 / Revised: 6 November 2018 / Accepted: 8 November 2018 / Published: 15 November 2018
(This article belongs to the Special Issue Electrochemical Immunosensor)
In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of β-lactoglobulin. Detection of this protein from cow’s milk was performed by a sandwich immunoassay onto printed carbon nanotube electrodes. The electrodes were printed using a carbon nanotube ink modified with polystyrene beads containing a high amount of carboxylic groups for protein immobilization. This strategy showed enhanced sensing performance compared to the use of oxidative treatments for the functionalization of electrodes. These electrodes showed an excellent electrochemical behavior, and proteins could be immobilized on their surface via the carbodiimide reaction. These antibody-immobilized carbon nanotube electrodes allowed for the detection of β-lactoglobulin in sub-ppm concentrations. View Full-Text
Keywords: electrochemical biosensor; carbon nanotubes; food allergen; β-lactoglobulin electrochemical biosensor; carbon nanotubes; food allergen; β-lactoglobulin
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MDPI and ACS Style

Molinari, J.; Florez, L.; Medrano, A.; Monsalve, L.; Ybarra, G. Electrochemical Determination of β-Lactoglobulin Employing a Polystyrene Bead-Modified Carbon Nanotube Ink. Biosensors 2018, 8, 109.

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