Next Article in Journal
Bees as Biosensors: Chemosensory Ability, Honey Bee Monitoring Systems, and Emergent Sensor Technologies Derived from the Pollinator Syndrome
Next Article in Special Issue
Asymmetric Mach–Zehnder Interferometer Based Biosensors for Aflatoxin M1 Detection
Previous Article in Journal
Biocompatible Hydrogels for Microarray Cell Printing and Encapsulation
Previous Article in Special Issue
Smart Textile Based on Fiber Bragg Grating Sensors for Respiratory Monitoring: Design and Preliminary Trials
Open AccessArticle

Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates

1
Centre for Microscopy and Imaging, National University Ireland, University Road, Galway, Ireland
2
School of Physics, National University Ireland, University Road, Galway, Ireland
3
Bio-Optical Imaging Group, Singapore Bioimaging Consortium, Agency for Science Technology and Research (A*STAR), 11 Biopolis Way, #02-02 Helios 138667, Singapore
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Yuliya Semenova
Biosensors 2015, 5(4), 664-677; https://doi.org/10.3390/bios5040664
Received: 3 September 2015 / Revised: 8 October 2015 / Accepted: 20 October 2015 / Published: 28 October 2015
(This article belongs to the Special Issue Optical Sensors for Biomedical Applications)
In this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering (SERRS) immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate the influence of varying concentrations of p53 and EGFR on the Raman spectra. Perturbations in the spectra are due to the influence of protein–antibody binding on Raman linker molecules and are attributed to small changes in localised mechanical stress, which are enhanced by SERRS. These influences are greatest for peaks due to the C-S functional group and the Full Width Half Maximum (FWHM) was found to be inversely proportional to protein concentration. View Full-Text
Keywords: Raman spectroscopy; protein sensing; surface-enhanced Raman scattering; biomarker Raman spectroscopy; protein sensing; surface-enhanced Raman scattering; biomarker
Show Figures

Figure 1

MDPI and ACS Style

Owens, P.; Phillipson, N.; Perumal, J.; O’Connor, G.M.; Olivo, M. Sensing of p53 and EGFR Biomarkers Using High Efficiency SERS Substrates. Biosensors 2015, 5, 664-677.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop