Next Article in Journal
Modified Electrodes Used for Electrochemical Detection of Metal Ions in Environmental Analysis
Next Article in Special Issue
Development of Functional Fluorescent Molecular Probes for the Detection of Biological Substances
Previous Article in Journal
Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time
Previous Article in Special Issue
A Signal-On Fluorosensor Based on Quench-Release Principle for Sensitive Detection of Antibiotic Rapamycin
Open AccessReview

Total Internal Reflection Fluorescence Quantification of Receptor Pharmacology

by Ye Fang
Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, NY 14831, USA
Academic Editor: A. Sheila Holmes-Smith
Biosensors 2015, 5(2), 223-240; https://doi.org/10.3390/bios5020223
Received: 22 March 2015 / Revised: 15 April 2015 / Accepted: 17 April 2015 / Published: 27 April 2015
(This article belongs to the Special Issue Fluorescence Based Sensing Technologies)
Total internal reflection fluorescence (TIRF) microscopy has been widely used as a single molecule imaging technique to study various fundamental aspects of cell biology, owing to its ability to selectively excite a very thin fluorescent volume immediately above the substrate on which the cells are grown. However, TIRF microscopy has found little use in high content screening due to its complexity in instrumental setup and experimental procedures. Inspired by the recent demonstration of label-free evanescent wave biosensors for cell phenotypic profiling and drug screening with high throughput, we had hypothesized and demonstrated that TIRF imaging is also amenable to receptor pharmacology profiling. This paper reviews key considerations and recent applications of TIRF imaging for pharmacology profiling. View Full-Text
Keywords: evanescent wave; receptor pharmacology; resonant waveguide grating; single molecule analysis; surface plasmon resonance; total internal reflection fluorescence evanescent wave; receptor pharmacology; resonant waveguide grating; single molecule analysis; surface plasmon resonance; total internal reflection fluorescence
Show Figures

Figure 1

MDPI and ACS Style

Fang, Y. Total Internal Reflection Fluorescence Quantification of Receptor Pharmacology. Biosensors 2015, 5, 223-240.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop