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Open AccessArticle

Label-Free Imaging and Biochemical Characterization of Bovine Sperm Cells

1
Institute for Microelectronics and Microsystems, National Research Council, Via P. Castellino, 111, 80131 Naples, Italy
2
Rowland Institute, Harvard University, Cambridge, MA 02142, USA
3
Institute of Protein Biochemistry, National Research Council, Via P. Castellino, 111, 80131 Naples, Italy
*
Authors to whom correspondence should be addressed.
Academic Editor: Andrew M. Shaw
Biosensors 2015, 5(2), 141-157; https://doi.org/10.3390/bios5020141
Received: 16 January 2015 / Revised: 18 February 2015 / Accepted: 24 March 2015 / Published: 1 April 2015
(This article belongs to the Special Issue Label-Free Biosensors: Exploring the Field)
A full label-free morphological and biochemical characterization is desirable to select spermatozoa during preparation for artificial insemination. In order to study these fundamental parameters, we take advantage of two attractive techniques: digital holography (DH) and Raman spectroscopy (RS). DH presents new opportunities for studying morphological aspect of cells and tissues non-invasively, quantitatively and without the need for staining or tagging, while RS is a very specific technique allowing the biochemical analysis of cellular components with a spatial resolution in the sub-micrometer range. In this paper, morphological and biochemical bovine sperm cell alterations were studied using these techniques. In addition, a complementary DH and RS study was performed to identify X- and Y-chromosome-bearing sperm cells. We demonstrate that the two techniques together are a powerful and highly efficient tool elucidating some important criterions for sperm morphological selection and sex-identification, overcoming many of the limitations associated with existing protocols. View Full-Text
Keywords: spermatozoa; biophotonics; sex-identification; digital holography microscopy; Raman spectroscopy; Raman imaging spermatozoa; biophotonics; sex-identification; digital holography microscopy; Raman spectroscopy; Raman imaging
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MDPI and ACS Style

Ferrara, M.A.; Di Caprio, G.; Managò, S.; De Angelis, A.; Sirleto, L.; Coppola, G.; De Luca, A.C. Label-Free Imaging and Biochemical Characterization of Bovine Sperm Cells. Biosensors 2015, 5, 141-157. https://doi.org/10.3390/bios5020141

AMA Style

Ferrara MA, Di Caprio G, Managò S, De Angelis A, Sirleto L, Coppola G, De Luca AC. Label-Free Imaging and Biochemical Characterization of Bovine Sperm Cells. Biosensors. 2015; 5(2):141-157. https://doi.org/10.3390/bios5020141

Chicago/Turabian Style

Ferrara, Maria A.; Di Caprio, Giuseppe; Managò, Stefano; De Angelis, Annalisa; Sirleto, Luigi; Coppola, Giuseppe; De Luca, Anna C. 2015. "Label-Free Imaging and Biochemical Characterization of Bovine Sperm Cells" Biosensors 5, no. 2: 141-157. https://doi.org/10.3390/bios5020141

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