Biconically Tapered Fiber Optic Probes for Rapid Label-Free Immunoassays†
Department of Physics and Astronomy, University of California, Los Angeles, 475 Portola Plaza, Los Angeles, CA 90095, USA
Department of Plant and Microbial Biology, University of California, Berkeley, 565 Li Ka Shing Center, Berkeley, CA 94720, USA
Department of Biological Sciences, California State Polytechnic University, Pomona, 3801 W Temple Ave, Pomona, CA 91768, USA
Department of Physics and Astronomy, California State Polytechnic University, Pomona, 3801 W Temple Ave, Pomona, CA 91768, USA
Department of Physics, Mus Alparslan University, Istasyon Cad. 49100, Mus, Turkey
Department of Biology, Mus Alparslan University, Istasyon Cad. 49100, Mus, Turkey
Department of Animal and Veterinary Sciences, California State Polytechnic University, Pomona, 3801 W Temple Ave, Pomona, CA 91768, USA
Based on “Miller, J.; Castaneda, A.; Lee, K.H.; Sanchez, M.; Murinda, S.; Lin, W.-J.; Salik, E. Biconically Tapered Fiber Optic Dip Probe for Rapid Label—Free Immunoassays. In Proceedings of Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV Israel Gannot, San Francisco, CA, USA, 1 February 2014”.
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Andrew M. Shaw
Received: 23 January 2015 / Revised: 16 March 2015 / Accepted: 20 March 2015 / Published: 1 April 2015
We report use of U-shaped biconically tapered optical fibers (BTOF) as probes for label-free immunoassays. The tapered regions of the sensors were functionalized by immobilization of immunoglobulin-G (Ig-G) and tested for detection of anti-IgG at concentrations of 50 ng/mL to 50 µg/mL. Antibody-antigen reaction creates a biological nanolayer modifying the waveguide structure leading to a change in the sensor signal, which allows real-time monitoring. The kinetics of the antibody (mouse Ig-G)-antigen (rabbit anti-mouse IgG) reactions was studied. Hydrofluoric acid treatment makes the sensitive region thinner to enhance sensitivity, which we confirmed by experiments and simulations. The limit of detection for the sensor was estimated to be less than 50 ng/mL. Utilization of the rate of the sensor peak shift within the first few minutes of the antibody-antigen reaction is proposed as a rapid protein detection method.
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Miller, J.; Castaneda, A.; Lee, K.H.; Sanchez, M.; Ortiz, A.; Almaz, E.; Almaz, Z.T.; Murinda, S.; Lin, W.-J.; Salik, E. Biconically Tapered Fiber Optic Probes for Rapid Label-Free Immunoassays. Biosensors 2015, 5, 158-171.
Miller J, Castaneda A, Lee KH, Sanchez M, Ortiz A, Almaz E, Almaz ZT, Murinda S, Lin W-J, Salik E. Biconically Tapered Fiber Optic Probes for Rapid Label-Free Immunoassays. Biosensors. 2015; 5(2):158-171.
Miller, John; Castaneda, Angelica; Lee, Kun H.; Sanchez, Martin; Ortiz, Adrian; Almaz, Ekrem; Almaz, Zuleyha T.; Murinda, Shelton; Lin, Wei-Jen; Salik, Ertan. 2015. "Biconically Tapered Fiber Optic Probes for Rapid Label-Free Immunoassays." Biosensors 5, no. 2: 158-171.
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