Highly Sensitive Detection of Hydrogen Peroxide in Cancer Tissue Based on 3D Reduced Graphene Oxide–MXene–Multi-Walled Carbon Nanotubes Electrode

Yu, Shuai-Qun; Li, Pan; Li, Hao-Jie; Shang, Ling-Jun; Guo, Rui; Sun, Xu-Ming; Ren, Qiong-Qiong

doi:10.3390/bios14060261

Open AccessArticle

Highly Sensitive Detection of Hydrogen Peroxide in Cancer Tissue Based on 3D Reduced Graphene Oxide–MXene–Multi-Walled Carbon Nanotubes Electrode

by

Shuai-Qun Yu

,

Pan Li

,

Hao-Jie Li

,

Ling-Jun Shang

,

Rui Guo

,

Xu-Ming Sun

^*

and

Qiong-Qiong Ren

^*

School of Medical Engineering, Xinxiang Medical University, Xinxiang 453003, China

^*

Authors to whom correspondence should be addressed.

Biosensors 2024, 14(6), 261; https://doi.org/10.3390/bios14060261

Submission received: 15 April 2024 / Revised: 11 May 2024 / Accepted: 19 May 2024 / Published: 21 May 2024

(This article belongs to the Special Issue Application of Biosensors in Cell or Tissue Analysis)

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Abstract

Hydrogen peroxide (H₂O₂) is a signaling molecule that has the capacity to control a variety of biological processes in organisms. Cancer cells release more H₂O₂ during abnormal tumor growth. There has been a considerable amount of interest in utilizing H₂O₂ as a biomarker for the diagnosis of cancer tissue. In this study, an electrochemical sensor for H₂O₂ was constructed based on 3D reduced graphene oxide (rGO), MXene (Ti₃C₂), and multi-walled carbon nanotubes (MWCNTs) composite. Three-dimensional (3D) rGO–Ti₃C₂–MWCNTs sensor showed good linearity for H₂O₂ in the ranges of 1–60 μM and 60 μM–9.77 mM at a working potential of −0.25 V, with sensitivities of 235.2 µA mM⁻¹ cm⁻² and 103.8 µA mM⁻¹ cm⁻², respectively, and a detection limit of 0.3 µM (S/N = 3). The sensor exhibited long-term stability, good repeatability, and outstanding immunity to interference. In addition, the modified electrode was employed to detect real-time H₂O₂ release from cancer cells and cancer tissue ex vivo.

Keywords: H₂O₂; rGO; MXene; MWCNTs; real-time detection; cancer tissue

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MDPI and ACS Style

Yu, S.-Q.; Li, P.; Li, H.-J.; Shang, L.-J.; Guo, R.; Sun, X.-M.; Ren, Q.-Q. Highly Sensitive Detection of Hydrogen Peroxide in Cancer Tissue Based on 3D Reduced Graphene Oxide–MXene–Multi-Walled Carbon Nanotubes Electrode. Biosensors 2024, 14, 261. https://doi.org/10.3390/bios14060261

AMA Style

Yu S-Q, Li P, Li H-J, Shang L-J, Guo R, Sun X-M, Ren Q-Q. Highly Sensitive Detection of Hydrogen Peroxide in Cancer Tissue Based on 3D Reduced Graphene Oxide–MXene–Multi-Walled Carbon Nanotubes Electrode. Biosensors. 2024; 14(6):261. https://doi.org/10.3390/bios14060261

Chicago/Turabian Style

Yu, Shuai-Qun, Pan Li, Hao-Jie Li, Ling-Jun Shang, Rui Guo, Xu-Ming Sun, and Qiong-Qiong Ren. 2024. "Highly Sensitive Detection of Hydrogen Peroxide in Cancer Tissue Based on 3D Reduced Graphene Oxide–MXene–Multi-Walled Carbon Nanotubes Electrode" Biosensors 14, no. 6: 261. https://doi.org/10.3390/bios14060261

APA Style

Yu, S.-Q., Li, P., Li, H.-J., Shang, L.-J., Guo, R., Sun, X.-M., & Ren, Q.-Q. (2024). Highly Sensitive Detection of Hydrogen Peroxide in Cancer Tissue Based on 3D Reduced Graphene Oxide–MXene–Multi-Walled Carbon Nanotubes Electrode. Biosensors, 14(6), 261. https://doi.org/10.3390/bios14060261

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Highly Sensitive Detection of Hydrogen Peroxide in Cancer Tissue Based on 3D Reduced Graphene Oxide–MXene–Multi-Walled Carbon Nanotubes Electrode

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