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Volume 13
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Article

Novel Cytochrome P450-3A4 Enzymatic Nanobiosensor for Lapatinib (a Breast Cancer Drug) Developed on a Poly(anilino-co-4-aminobenzoic Acid-Green-Synthesised Indium Nanoparticle) Platform

by
Jaymi Leigh January
1,*,
Ziyanda Zamaswazi Tshobeni
1,
Nokwanda Precious Pearl Ngema
1,
Abongile Nwabisa Jijana
2,
Emmanuel Iheanyichukwu Iwuoha
1,
Takalani Mulaudzi
3,
Samantha Fiona Douman
4,* and
Rachel Fanelwa Ajayi
1
1
SensorLab (UWC Sensor Laboratories), University of the Western Cape, Private Bag X17, Bellville, Cape Town 7535, South Africa
2
Nanotechnology Innovation Centre, Advanced Materials Division, Mintek, Private Bag X3015, Randburg, Johannesburg 2125, South Africa
3
Department of Biotechnology, University of the Western Cape, Private Bag X17, Bellville, Cape Town 7535, South Africa
4
Department of Chemistry, University of Cape Town, Private Bag X3, Rondebosch, Cape Town 7701, South Africa
*
Authors to whom correspondence should be addressed.
Biosensors 2023, 13(9), 897; https://doi.org/10.3390/bios13090897
Submission received: 27 July 2023 / Revised: 15 September 2023 / Accepted: 18 September 2023 / Published: 21 September 2023
(This article belongs to the Special Issue Advanced Materials for Electrochemical Sensors and Biosensors Development)
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Abstract

Breast cancer (BC) is one of the most common types of cancer disease worldwide and it accounts for thousands of deaths annually. Lapatinib is among the preferred drugs for the treatment of breast cancer. Possible drug toxicity effects of lapatinib can be controlled by real-time determination of the appropriate dose for a patient at the point of care. In this study, a novel highly sensitive polymeric nanobiosensor for lapatinib is presented. A composite of poly(anilino-co-4-aminobenzoic acid) co-polymer {poly(ANI-co-4-ABA)} and coffee extract-based green-synthesized indium nanoparticles (InNPs) was used to develop the sensor platform on a screen-printed carbon electrode (SPCE), i.e., SPCE||poly(ANI-co-4-ABA-InNPs). Cytochrome P450-3A4 (CYP3A4) enzyme and polyethylene glycol (PEG) were incorporated on the modified platform to produce the SPCE||poly(ANI-co-4-ABA-InNPs)|CYP3A4|PEG lapatinib nanobiosensor. Experiments for the determination of the electrochemical response characteristics of the nanobiosensor were performed with cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The nanobiosensor calibration for 0–100 ng/mL lapatinib was linear and gave limit of detection (LOD) values of 13.21 ng/mL lapatinib and 18.6 ng/mL lapatinib in physiological buffer and human serum, respectively. The LOD values are much lower than the peak plasma concentration (Cmax) of lapatinib (2.43 µg/mL), which is attained 4 h after the administration of a daily dose of 1250 mg lapatinib. The electrochemical nanobiosensor also exhibited excellent anti-interference performance and stability.
Keywords: biosensor; breast cancer drug (lapatinib); cytochrome P450-3A4; green-synthesized indium nanoparticles; polyaniline biosensor; breast cancer drug (lapatinib); cytochrome P450-3A4; green-synthesized indium nanoparticles; polyaniline
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MDPI and ACS Style

January, J.L.; Tshobeni, Z.Z.; Ngema, N.P.P.; Jijana, A.N.; Iwuoha, E.I.; Mulaudzi, T.; Douman, S.F.; Ajayi, R.F. Novel Cytochrome P450-3A4 Enzymatic Nanobiosensor for Lapatinib (a Breast Cancer Drug) Developed on a Poly(anilino-co-4-aminobenzoic Acid-Green-Synthesised Indium Nanoparticle) Platform. Biosensors 2023, 13, 897. https://doi.org/10.3390/bios13090897

AMA Style

January JL, Tshobeni ZZ, Ngema NPP, Jijana AN, Iwuoha EI, Mulaudzi T, Douman SF, Ajayi RF. Novel Cytochrome P450-3A4 Enzymatic Nanobiosensor for Lapatinib (a Breast Cancer Drug) Developed on a Poly(anilino-co-4-aminobenzoic Acid-Green-Synthesised Indium Nanoparticle) Platform. Biosensors. 2023; 13(9):897. https://doi.org/10.3390/bios13090897

Chicago/Turabian Style

January, Jaymi Leigh, Ziyanda Zamaswazi Tshobeni, Nokwanda Precious Pearl Ngema, Abongile Nwabisa Jijana, Emmanuel Iheanyichukwu Iwuoha, Takalani Mulaudzi, Samantha Fiona Douman, and Rachel Fanelwa Ajayi. 2023. "Novel Cytochrome P450-3A4 Enzymatic Nanobiosensor for Lapatinib (a Breast Cancer Drug) Developed on a Poly(anilino-co-4-aminobenzoic Acid-Green-Synthesised Indium Nanoparticle) Platform" Biosensors 13, no. 9: 897. https://doi.org/10.3390/bios13090897

APA Style

January, J. L., Tshobeni, Z. Z., Ngema, N. P. P., Jijana, A. N., Iwuoha, E. I., Mulaudzi, T., Douman, S. F., & Ajayi, R. F. (2023). Novel Cytochrome P450-3A4 Enzymatic Nanobiosensor for Lapatinib (a Breast Cancer Drug) Developed on a Poly(anilino-co-4-aminobenzoic Acid-Green-Synthesised Indium Nanoparticle) Platform. Biosensors, 13(9), 897. https://doi.org/10.3390/bios13090897

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