Odorant Binding Causes Cytoskeletal Rearrangement, Leading to Detectable Changes in Endothelial and Epithelial Barrier Function and Micromotion
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells
2.2. Odorants
2.3. Electric-Cell Substrate Impedance Sensing (ECIS)
2.4. Micromotion
2.5. cAMP Activity
2.6. Actin and Tubulin Staining
2.7. Fluorescence Quantification
2.8. LDH Release
2.9. Statistics
3. Results and Discussion
3.1. Detection of Odorants Using Cellular Resistance
3.2. Determination of cAMP Activity after Odorant Exposure
3.3. Characterization of Cytoskeletal Organization after Odorant Exposure
3.4. Detection of Odorants Using Cellular Micromotion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Curtis, T.M.; Nilon, A.M.; Greenberg, A.J.; Besner, M.; Scibek, J.J.; Nichols, J.A.; Huie, J.L. Odorant Binding Causes Cytoskeletal Rearrangement, Leading to Detectable Changes in Endothelial and Epithelial Barrier Function and Micromotion. Biosensors 2023, 13, 329. https://doi.org/10.3390/bios13030329
Curtis TM, Nilon AM, Greenberg AJ, Besner M, Scibek JJ, Nichols JA, Huie JL. Odorant Binding Causes Cytoskeletal Rearrangement, Leading to Detectable Changes in Endothelial and Epithelial Barrier Function and Micromotion. Biosensors. 2023; 13(3):329. https://doi.org/10.3390/bios13030329
Chicago/Turabian StyleCurtis, Theresa M., Annabella M. Nilon, Anthony J. Greenberg, Matthew Besner, Jacob J. Scibek, Jennifer A. Nichols, and Janet L. Huie. 2023. "Odorant Binding Causes Cytoskeletal Rearrangement, Leading to Detectable Changes in Endothelial and Epithelial Barrier Function and Micromotion" Biosensors 13, no. 3: 329. https://doi.org/10.3390/bios13030329