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Volume 12
Issue 6
10.3390/bios12060423
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Article

Development of Fluorescent Aptasensors Based on G-Quadruplex Quenching Ability for Ochratoxin A and Potassium Ions Detection

by
Cheng Yang
1,*,
Xiaolin Chu
1,
Li Zeng
2,
Amina Rhouati
3,
Fathimath Abbas
1,
Shengnan Cui
1 and
Daiqin Lin
2,*
1
State Key Laboratory of Fine Chemicals, Department of Chemistry, School of Chemical Engineering, Dalian University of Technology, Dalian 116024, China
2
Institute of Inspection and Testing for Industrial Products of Jiangxi General Institute of Testing, Nanchang 330052, China
3
Bioengineering Laboratory, Higher National School of Biotechnology, Constantine 25100, Algeria
*
Authors to whom correspondence should be addressed.
Biosensors 2022, 12(6), 423; https://doi.org/10.3390/bios12060423
Submission received: 4 May 2022 / Revised: 8 June 2022 / Accepted: 10 June 2022 / Published: 16 June 2022
(This article belongs to the Special Issue Smart and Multifunctional Nanomaterials and Applications for Food Safety)
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Abstract

G-quadruplexes have received significant attention in aptasensing due to their structural polymorphisms and unique binding properties. In this work, we exploited the fluorescence-quenching properties of G-quadruplex to develop a simple, fast, and sensitive platform for fluorescence detection of ochratoxin A (OTA) and potassium ions (K+) with a label-free fluorophore and quencher strategy. The quenching ability of G-quadruplex was confirmed during the recognition process after the formation of the G-quadruplex structure and the quenching of the labeled fluorescein fluorophore (FAM). The fluorescence-quenching mechanism was studied by introducing specific ligands of G-quadruplex to enhance the quenching effect, to show that this phenomenon is due to photo-induced electron transfer. The proposed fluorescence sensor based on G-quadruplex quenching showed excellent selectivity with a low detection limit of 0.19 nM and 0.24 µM for OTA and K+, respectively. Moreover, we demonstrated that our detection method enables accurate concentration determination of real samples with the prospect of practical application. Therefore, G-quadruplexes can be excellent candidates as quenchers, and the strategy implemented in the study can be extended to an aptasensor with G-quadruplex.
Keywords: G-quadruplex; label-free; fluorescence aptasensor; photo-induced electron transfer G-quadruplex; label-free; fluorescence aptasensor; photo-induced electron transfer

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MDPI and ACS Style

Yang, C.; Chu, X.; Zeng, L.; Rhouati, A.; Abbas, F.; Cui, S.; Lin, D. Development of Fluorescent Aptasensors Based on G-Quadruplex Quenching Ability for Ochratoxin A and Potassium Ions Detection. Biosensors 2022, 12, 423. https://doi.org/10.3390/bios12060423

AMA Style

Yang C, Chu X, Zeng L, Rhouati A, Abbas F, Cui S, Lin D. Development of Fluorescent Aptasensors Based on G-Quadruplex Quenching Ability for Ochratoxin A and Potassium Ions Detection. Biosensors. 2022; 12(6):423. https://doi.org/10.3390/bios12060423

Chicago/Turabian Style

Yang, Cheng, Xiaolin Chu, Li Zeng, Amina Rhouati, Fathimath Abbas, Shengnan Cui, and Daiqin Lin. 2022. "Development of Fluorescent Aptasensors Based on G-Quadruplex Quenching Ability for Ochratoxin A and Potassium Ions Detection" Biosensors 12, no. 6: 423. https://doi.org/10.3390/bios12060423

APA Style

Yang, C., Chu, X., Zeng, L., Rhouati, A., Abbas, F., Cui, S., & Lin, D. (2022). Development of Fluorescent Aptasensors Based on G-Quadruplex Quenching Ability for Ochratoxin A and Potassium Ions Detection. Biosensors, 12(6), 423. https://doi.org/10.3390/bios12060423

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