Next Article in Journal
Accuracy Assessment of the GlucoMen® Day CGM System in Individuals with Type 1 Diabetes: A Pilot Study
Next Article in Special Issue
A Lab-Made E-Nose-MOS Device for Assessing the Bacterial Growth in a Solid Culture Medium
Previous Article in Journal
A Label-Free Electrical Impedance Spectroscopy for Detection of Clusters of Extracellular Vesicles Based on Their Unique Dielectric Properties
Previous Article in Special Issue
Digital E. coli Counter: A Microfluidics and Computer Vision-Based DNAzyme Method for the Isolation and Specific Detection of E. coli from Water Samples
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biosensors
Volume 12
Issue 2
10.3390/bios12020105
biosensors-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Selective Detection of Legionella pneumophila Serogroup 1 and 5 with a Digital Photocorrosion Biosensor Using Antimicrobial Peptide-Antibody Sandwich Strategy

by
M. Amirul Islam
1,
Walid M. Hassen
1,
Ishika Ishika
1,
Azam F. Tayabali
2 and
Jan J. Dubowski
1,*
1
Laboratory for Quantum Semiconductors and Photon-Based BioNanotechnology, Interdisciplinary Institute for Technological Innovation (3IT), CNRS UMI-3463, Department of Electrical and Computer Engineering, Université de Sherbrooke, 3000, Boul. de l’Université, Sherbrooke, QC J1K 0A5, Canada
2
Biotechnology Laboratory, Environmental Health Science and Research Bureau, Healthy Environments and Consumer Safety Branch, Environmental Health Centre, Health Canada, Ottawa, ON K1A 0K9, Canada
*
Author to whom correspondence should be addressed.
Biosensors 2022, 12(2), 105; https://doi.org/10.3390/bios12020105
Submission received: 5 December 2021 / Revised: 2 February 2022 / Accepted: 2 February 2022 / Published: 9 February 2022
(This article belongs to the Special Issue Biosensors for Bacterial and Viral Detection)
Browse Figures
Versions Notes

Abstract

Rapid detection of Legionella pneumophila (L. pneumophila) is important for monitoring the presence of these bacteria in water sources and preventing the transmission of the Legionnaires’ disease. We report improved biosensing of L. pneumophila with a digital photocorrosion (DIP) biosensor functionalized with an innovative structure of cysteine-modified warnericin antimicrobial peptides for capturing bacteria that are subsequently decorated with anti-L. pneumophila polyclonal antibodies (pAbs). The application of peptides for the operation of a biosensing device was enabled by the higher bacterial-capture efficiency of peptides compared to other traditional ligands, such as those based on antibodies or aptamers. At the same time, the significantly stronger affinity of pAbs decorating the L. pneumophila serogroup-1 (SG-1) compared to serogroup-5 (SG-5) allowed for the selective detection of L. pneumophila SG-1 at 50 CFU/mL. The results suggest that the attractive sensitivity of the investigated sandwich method is related to the flow of an extra electric charge between the pAb and a charge-sensing DIP biosensor. The method has the potential to offer highly specific and sensitive detection of L. pneumophila as well as other pathogenic bacteria and viruses.
Keywords: cysteine-modified warnericin RK; antimicrobial peptides; anti-Legionella pneumophila polyclonal antibody; digital photocorrosion biosensor; GaAs/AlGaAs nanoheterostructures cysteine-modified warnericin RK; antimicrobial peptides; anti-Legionella pneumophila polyclonal antibody; digital photocorrosion biosensor; GaAs/AlGaAs nanoheterostructures

Share and Cite

MDPI and ACS Style

Islam, M.A.; Hassen, W.M.; Ishika, I.; Tayabali, A.F.; Dubowski, J.J. Selective Detection of Legionella pneumophila Serogroup 1 and 5 with a Digital Photocorrosion Biosensor Using Antimicrobial Peptide-Antibody Sandwich Strategy. Biosensors 2022, 12, 105. https://doi.org/10.3390/bios12020105

AMA Style

Islam MA, Hassen WM, Ishika I, Tayabali AF, Dubowski JJ. Selective Detection of Legionella pneumophila Serogroup 1 and 5 with a Digital Photocorrosion Biosensor Using Antimicrobial Peptide-Antibody Sandwich Strategy. Biosensors. 2022; 12(2):105. https://doi.org/10.3390/bios12020105

Chicago/Turabian Style

Islam, M. Amirul, Walid M. Hassen, Ishika Ishika, Azam F. Tayabali, and Jan J. Dubowski. 2022. "Selective Detection of Legionella pneumophila Serogroup 1 and 5 with a Digital Photocorrosion Biosensor Using Antimicrobial Peptide-Antibody Sandwich Strategy" Biosensors 12, no. 2: 105. https://doi.org/10.3390/bios12020105

APA Style

Islam, M. A., Hassen, W. M., Ishika, I., Tayabali, A. F., & Dubowski, J. J. (2022). Selective Detection of Legionella pneumophila Serogroup 1 and 5 with a Digital Photocorrosion Biosensor Using Antimicrobial Peptide-Antibody Sandwich Strategy. Biosensors, 12(2), 105. https://doi.org/10.3390/bios12020105

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop