Label-Free Myoglobin Biosensor Based on Pure and Copper-Doped Titanium Dioxide Nanomaterials
Abstract
:1. Introduction
2. Experimental Details
2.1. Materials
2.2. Synthesis of Pure and Cu-Doped TiO2 Nanomaterials
2.3. Characterizations of Pure and Cu-Doped TiO2 Nanomaterials
2.4. Fabrication and Characterizations of Myoglobin (Mb) Biosensors Using Pure and Cu-Doped TiO2 Nanomaterials
3. Results and Discussion
3.1. Characterizations and Properties of Pure and Cu-Doped TiO2 Nanomaterials
3.2. Fabrication and Characterizations of Mb Biosensor Based on Pure and Cu-Doped TiO2 Nanomaterials
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
- McDonnell, B.; Hearty, S.; Leonard, P.; O’Kennedy, R. Cardiac biomarkers and the case for point-of-care testing. Clin. Biochem. 2009, 42, 549–561. [Google Scholar] [CrossRef] [PubMed]
- Mehta, P.K.; Wei, J.; Wenger, N.K. Ischemic heart disease in women: A focus on risk factors. Trends Cardiovasc. Med. 2014, 25, 140–151. [Google Scholar] [CrossRef] [Green Version]
- Tang, X.; Zhu, Y.; Guan, W.; Zhou, W.; Wei, P. Advances in nanosensors for cardiovascular disease detection. Life Sci. 2022, 305, 120733. [Google Scholar] [CrossRef] [PubMed]
- Qureshi, A.; Gurbuz, Y.; Niazi, J.H. Biosensors for cardiac biomarkers detection: A review. Sens. Actuators B Chem. 2012, 171–172, 62–76. [Google Scholar] [CrossRef] [Green Version]
- Anderson, J.L.; Morrow, D.A. Acute myocardial infarction. N. Engl. J. Med. 2017, 376, 2053–2064. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Danese, E.; Montagnana, M. An historical approach to the diagnostic biomarkers of acute coronary syndrome. Ann. Transl. Med. 2016, 4, 194. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Morrow, D.A.; Cannon, C.P.; Jesse, R.L.; Newby, L.K.; Ravkilde, J.; Storrow, A.B.; Wu, A.H.; Christenson, R.H. National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines: Clinical Characteristics and Utilization of Biochemical Markers in Acute Coronary Syndromes. Circulation 2007, 115, e356–e375. [Google Scholar]
- Avila, B.; Gomez, V.; Campuzano, S.; Pedrero, M.; Pingarrón, J. Disposable ampemmetric magneto for the sensitive detection of the cardiac hiomarker amino-terminal pro-B-type natriuretic peptide in human serum. Anal. Chim. Acta 2013, 784, 18–24. [Google Scholar]
- Kwon, Y.-C.; Kim, M.-G.; Kim, E.-M.; Shin, Y.-B.; Lee, S.-K.; Lee, S.D.; Cho, M.-J.; Ro, H.-S. Development of a surface plasmon resonance-based immunosensor for the rapid detection of cardiac troponin I. Biotechnol. Lett. 2011, 33, 921–927. [Google Scholar] [CrossRef]
- Lee, J.; Lee, Y.; Park, J.; Seo, H.; Lee, T.; Lee, W.; Kim, S.; Hahn, Y.K.; Jung, J.; Kim, S.; et al. Sensitive and reproducible detection of cardiac troponin I in human plasma using a surface acoustic wave immunosensor. Sens. Actuators B Chem. 2013, 178, 19–25. [Google Scholar] [CrossRef]
- Tian, L.; Heyduk, T. Antigen Peptide-Based Immunosensors for Rapid Detection of Antibodies and Antigens. Anal. Chem. 2009, 81, 5218–5225. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Järvenpää, M.-L.; Kuningas, K.; Niemi, I.; Hedberg, P.; Ristiniemi, N.; Pettersson, K.; Lövgren, T. Rapid and sensitive cardiac troponin I immunoassay based on fluorescent europium(III)-chelate-dyed nanoparticles. Clin. Chim. Acta 2012, 414, 70–75. [Google Scholar] [CrossRef]
- Zhang, Q.; Prabhu, A.; San, A.; Al-Sharab, J.F.; Levon, K. A polyaniline based ultrasensitive potentiometric immunosensor for cardiac troponin complex detection. Biosens. Bioelectron. 2015, 72, 100–106. [Google Scholar] [CrossRef] [PubMed]
- Billah, M.; Hays, H.C.; Hodges, C.S.; Ponnambalam, S.; Vohra, R.; Millner, P.A. Mixed self-assembled monolayer (mSAM) based impedimetric immunosensors for cardiac troponin I (cTnI) and soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1). Sens. Actuators B Chem. 2012, 173, 361–366. [Google Scholar] [CrossRef]
- Jiang, W.; Rutherford, D.; Vuong, T.; Liu, H. Nanomaterials for treating cardiovascular diseases: A review. Bioact. Mater. 2017, 2, 185–198. [Google Scholar] [CrossRef]
- Nsabimana, A.; Ma, X.; Yuan, F.; Du, F.; Abdussalam, A.; Lou, B.; Xu, G. Nanomaterials-based Electrochemical Sensing of Cardiac Biomarkers for Acute Myocardial Infarction: Recent Progress. Electroanalysis 2018, 31, 177–187. [Google Scholar] [CrossRef]
- Hu, Q.; Fang, Z.; Ge, J.; Li, H. Nanotechnology for cardiovascular diseases. Innovation 2022, 3, 100214. [Google Scholar] [CrossRef]
- Shi, C.; Xie, H.; Ma, Y.; Yang, Z.; Zhang, J. Nanoscale Technologies in Highly Sensitive Diagnosis of Cardiovascular Diseases. Front. Bioeng. Biotechnol. 2020, 8, 531. [Google Scholar] [CrossRef]
- Li, F.; Yu, Y.; Cui, H.; Yang, D.; Bian, Z. Label-free electrochemiluminescence immunosensor for cardiac troponin I using luminol functionalized gold nanoparticles as a sensing platform. Analyst 2013, 138, 1844–1850. [Google Scholar] [CrossRef]
- Mishra, S.K.; Kumar, D.; Biradar, A.M. Rajesh Electrochemical impedance spectroscopy characterization of mercaptopropionic acid capped ZnS nanocrystal based bioelectrode for the detection of the cardiac biomarker—myoglobin. Bioelectrochemistry 2012, 88, 118–126. [Google Scholar] [CrossRef]
- Zhang, B.; Zhang, Y.; Liang, W.; Yu, X.; Tan, H.; Wang, G.; Li, A.; Jin, J.; Huang, L. Copper sulfide-functionalized molybdenum disulfide nanohybrids as nanoenzyme mimics for electrochemical immunoassay of myoglobin in cardiovascular disease. RSC Adv. 2017, 7, 2486–2493. [Google Scholar] [CrossRef] [Green Version]
- Ribeiro, J.; Pereira, C.; Silva, A.; Sales, M.G.F. Electrochemical detection of cardiac biomarker myoglobin using polyphenol as imprinted polymer receptor. Anal. Chim. Acta 2017, 981, 41–52. [Google Scholar] [CrossRef] [PubMed]
- Pakapongpan, S.; Palangsuntikul, R.; Surareungchai, W. Electrochemical sensors for hemoglobin and myoglobin detection based on methylene blue-multiwalled carbon nanotubes nanohybrid-modified glassy carbon electrode. Electrochim. Acta 2011, 56, 6831–6836. [Google Scholar] [CrossRef]
- Suprun, E.V.; Shilovskaya, A.L.; Lisitsa, A.V.; Bulko, T.V.; Shumyantseva, V.V.; Archakov, A.I. Electrochemical immunosensor based on metal nanoparticles for cardiac myoglobin detection in human blood plasma. Electroanalysis 2011, 23, 1051–1057. [Google Scholar] [CrossRef]
- Kumar, V.; Brent, J.R.; Shorie, M.; Kaur, H.; Chadha, G.; Thomas, A.G.; Lewis, E.A.; Rooney, A.P.; Nguyen, L.; Zhong, X.L.; et al. Nanostructured Aptamer-Functionalized Black Phosphorus Sensing Platform for Label-Free Detection of Myoglobin, a Cardiovascular Disease Biomarker. ACS Appl. Mater. Interfaces 2016, 8, 22860–22868. [Google Scholar] [CrossRef]
- Tuteja, S.K.; Chen, R.; Kukkar, M.; Song, C.K.; Mutreja, R.; Singh, S.; Paul, A.K.; Lee, H.; Kim, K.-H.; Deep, A.; et al. A label-free electrochemical immunosensor for the detection of cardiac marker using graphene quantum dots (GQDs). Biosens. Bioelectron. 2016, 86, 548–556. [Google Scholar] [CrossRef]
- Ren, X.; Zhang, Y.; Sun, Y.; Gao, L. Development of electrochemical impedance immunosensor for sensitive determination of myoglobin. Int. J. Electrochem. Sci. 2017, 12, 7765–7776. [Google Scholar] [CrossRef]
- Sun, L.; Li, W.; Wang, M.; Ding, W.; Ji, Y. Development of an electrochemical impedance immunosensor for myoglobin determination. Int. J. Electrochem. Sci. 2017, 7, 6170–6179. [Google Scholar] [CrossRef]
- Li, C.; Li, J.; Yang, X.; Gao, L.; Jing, L.; Ma, X. A label-free electrochemical aptasensor for sensitive myoglobin detection in meat. Sens. Actuators B Chem. 2017, 242, 1239–1245. [Google Scholar] [CrossRef]
- Al Fatease, A.; Haque, M.; Umar, A.; Ansari, S.; Alhamhoom, Y.; Muhsinah, A.; Mahnashi, M.; Guo, W.; Ansari, Z. Label-Free Electrochemical Sensor Based on Manganese Doped Titanium Dioxide Nanoparticles for Myoglobin Detection: Biomarker for Acute Myocardial Infarction. Molecules 2021, 26, 4252. [Google Scholar] [CrossRef]
- Masson, J.-F.; Obando, L.; Beaudoin, S.; Booksh, K. Sensitive and real-time fiber-optic-based surface plasmon resonance sensors for myoglobin and cardiac troponin I. Talanta 2004, 62, 865–870. [Google Scholar] [CrossRef] [PubMed]
- Haque, M.; Fouad, H.; Seo, H.-K.; Othman, A.Y.; Kulkarni, A.; Ansari, Z.A. Investigation of Mn Doped ZnO Nanoparticles Towards Ascertaining Myocardial Infarction Through an Electrochemical Detection of Myoglobin. IEEE Access 2020, 8, 164678–164692. [Google Scholar] [CrossRef]
- Wang, Y.; Han, M.; Ye, X.; Wu, K.; Wu, T.; Li, C. Voltammetric myoglobin sensor based on a glassy carbon electrode modified with a composite film consisting of carbon nanotubes and a molecularly imprinted polymerized ionic liquid. Microchim. Acta 2016, 184, 195–202. [Google Scholar] [CrossRef]
- Taghdisi, S.M.; Danesh, N.M.; Ramezani, M.; Emrani, A.S.; Abnous, K. A novel electrochemical aptasensor based on Y-shape structure of dual-aptamer-complementary strand conjugate for ultrasensitive detection of myoglobin. Biosens. Bioelectron. 2016, 80, 532–537. [Google Scholar] [CrossRef] [PubMed]
- Singh, S.; Tuteja, S.K.; Sillu, D.; Deep, A.; Suri, C.R. Gold nanoparticles-reduced graphene oxide based electrochemical immunosensor for the cardiac biomarker myoglobin. Mikrochim. Acta 2016, 183, 1729–1738. [Google Scholar] [CrossRef]
- Abdorahim, M.; Rabiee, M.; Alhosseini, S.N.; Tahriri, M.; Yazdanpanah, S.; Alavi, S.H.; Tayeb, L. Nanomaterials-based electrochemical immunosensors for cardiac troponin recognition: An illustrated review. TrAC Trends Anal. Chem. 2016, 82, 337–347. [Google Scholar] [CrossRef]
- Sharma, D.; Lee, J.; Shin, H. An electrochemical immunosensor based on a 3D carbon system consisting of a suspended mesh and substrate-bound interdigitated array nanoelectrodes for sensitive cardiac biomarker detection. Biosens. Bioelectron. 2018, 107, 10–16. [Google Scholar] [CrossRef]
- Tang, J.; Zhang, X.; Xiao, S.; Zeng, F. Application of TiO2 Nanotubes Gas Sensors in Online Monitoring of SF6 Insulated Equipment; INTECH Open Science Open Mind: London, UK, 2017. [Google Scholar] [CrossRef] [Green Version]
- Rajeswari, B.; Reddy, K.V.N.S.; Devi, S.A.; Madhavi, G.; Reddy, I.R.V.S. Determination of Uric Acid Using TiO2 Nanoparticles Modified Glassy Carbon Electrode. Biointerface Res. Appl. Chem. 2021, 12, 6058–6065. [Google Scholar] [CrossRef]
- Amiri, M.; Arshi, S. An Overview on Electrochemical Determination of Cholesterol. Electroanalysis 2020, 32, 1391–1407. [Google Scholar] [CrossRef]
- Azzouz, A.; Hejji, L.; Sonne, C.; Kim, K.; Kumar, V. Nanomaterial-based aptasensors as an efficient substitute for cardiovascular disease diagnosis: Future of smart biosensors. Biosens. Bioelectron. 2021, 193, 113617. [Google Scholar] [CrossRef]
- Cho, I.-H.; Kim, D.H.; Park, S. Electrochemical biosensors: Perspective on functional nanomaterials for on-site analysis. Biomater. Res. 2020, 24, 1–12. [Google Scholar] [CrossRef]
- Wu, S.; Weng, Z.; Liu, X.; Yeung, K.; Chu, P.K. Functionalized TiO2Based Nanomaterials for Biomedical Applications. Adv. Funct. Mater. 2014, 24, 5464–5481. [Google Scholar] [CrossRef]
- Chen, Y.; Liu, B.; Chen, Z.; Zuo, X. Innovative Electrochemical Sensor Using TiO2 Nanomaterials to Detect Phosphopeptides. Anal. Chem. 2021, 93, 10635–10643. [Google Scholar] [CrossRef]
- Bertel, L.; Miranda, D.A.; García-Martín, J.M. Nanostructured Titanium Dioxide Surfaces for Electrochemical Biosensing. Sensors 2021, 21, 6167. [Google Scholar] [CrossRef]
- Marvic, T.; Bencina, M.; Imani, R.; Junkar, I.; Valant, M.; Kralj-Iglic, V.; Iglic, A. Electrochemical Biosensors based on TiO2 Nanomaterials for Cancer Diagnosis. Adv. Bio-Membr. Lipid Self-Assem. 2018, 27, 63–105. [Google Scholar] [CrossRef]
- Arshad, M.K.; Adzhri, R.; Fathil, M.F.M.; Gopinath, S.C.B.; Ma, N.M.N. Field-Effect Transistor-Integration with TiO2 Nanoparticles for Sensing of Cardiac Troponin I Biomarker. J. Nanosci. Nanotechnol. 2018, 18, 5283–5291. [Google Scholar] [CrossRef]
- Soumit, S.; Mandal, K. Karthik Narayan and Aninda J. Bhattacharyya, Employing denaturation for rapid electrochemical detection of myoglobin using TiO2 nanotubes. J. Mater. Chem. B 2013, 1, 3051. [Google Scholar]
- Haque, M.; Fouad, H.; Seo, H.-K.; Alothman, O.Y.; Ansari, Z.A. Cu-Doped ZnO Nanoparticles as an Electrochemical Sensing Electrode for Cardiac Biomarker Myoglobin Detection. IEEE Sens. J. 2020, 20, 8820–8832. [Google Scholar] [CrossRef]
- Khan, R.; Pal, M.; Kuzikov, A.V.; Bulko, T.; Suprun, E.V.; Shumyantseva, V.V. Impedimetric immunosensor for detection of cardiovascular disorder risk biomarker. Mater. Sci. Eng. C 2016, 68, 52–58. [Google Scholar] [CrossRef]
- Bao, S.-J.; Li, C.M.; Zang, J.; Cui, X.-Q.; Qiao, Y.; Guo, J. New Nanostructured TiO2 for Direct Electrochemistry and Glucose Sensor Applications. Adv. Funct. Mater. 2008, 18, 591–599. [Google Scholar] [CrossRef]
- Jafari, S.; Mahyad, B.; Hashemzadeh, H.; Janfaza, S.; Gholikhani, T.; Tayebi, L. Biomedical Applications of TiO2 Nanostructures: Recent Advances. Int. J. Nanomed. 2020, 15, 3447–3470. [Google Scholar] [CrossRef] [PubMed]
- Viticoli, M.; Curulli, A.; Cusma, A.; Kaciulis, S.; Nunziante, S.; Pandolfi, L.; Valentini, F.; Padeletti, G. Third-generation biosensors based on TiO2 nanostructured films. Mater. Sci. Eng. C 2006, 26, 947–951. [Google Scholar] [CrossRef]
Samples | Peak Oxidation Potential (V) | Peak Reduction Potential (V) | Diffusion Coefficient (D) (×10−9 cm2/s) | Sensitivity (µAcm−2/nM) | LOD Value (pM) |
---|---|---|---|---|---|
S0 | 0.04–0.08 | −0.24, −0.28 | 0.76 | 23.43 | 153 |
S1 | 0.04–0.06 | −0.32–(−0.16) | 1.87 | 61.51 | 14 |
S2 | 0.08–0.04 | −0.30 | 1.19 | 42.45 | 32 |
S3 | 0.06 | −0.22 | 0.87 | 20.43 | 68 |
Sensing Matrix | Method | Linear range | LOD | Ref. |
---|---|---|---|---|
Anti-MYO/4-ATP SAM/Au | EIS | 350 ngmL−1–17.5 μgmL−1 | 5.5 ngmL−1 | [27] |
Ab-MYO/AuNps/APTES/ITO | EIS | 10 ngmL−1–1μgmL−1 | 2.7 ngmL−1 | [28] |
MBA/AuNps/RGD/GRCOOH/GCE | EIS | 0.0001–0.2 gL−1 | 26.3 ngmL−1 | [29] |
TiO2-Mn NPs/SPE | CV and EIS | 3 nM–15 nM | 0.013 nM | [30] |
Fiber-optics | SPR | 15–30 ngml−1 | 2.9 ngmL−1 | [31] |
ZnO-Mn NPs/SPE | CV and EIS | 0.024–2.4 ngmL−1 | 0.35 nM | [32] |
MIP/MWCNT/GCE | DPV | 1 μgmL−1–0.1 mgmL−1 | 0.17 μgmL−1 | [33] |
DApt/Exo I/Au | DPV | 0–1.4 μgmL−1 | 0.47 ngmL−1 | [34] |
AuNp@rGO/SPE | DPV | 1 ngmL−1–1400 ngmL−1 | 0.67 ngmL−1 | [35] |
ZnO-Cu NPs/SPE | CV and EIS | 3 nM–15 nM | 0.46 nM | [49] |
Cu-TiO2 NPs/SPE | CV and EIS | 3 nM–15 nM | S0:0.153 nM S1:0.014 nM S2: 0.032 nM S3:0.068 nM | This work |
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Umar, A.; Haque, M.; Ansari, S.G.; Seo, H.-K.; Ibrahim, A.A.; Alhamami, M.A.M.; Algadi, H.; Ansari, Z.A. Label-Free Myoglobin Biosensor Based on Pure and Copper-Doped Titanium Dioxide Nanomaterials. Biosensors 2022, 12, 1151. https://doi.org/10.3390/bios12121151
Umar A, Haque M, Ansari SG, Seo H-K, Ibrahim AA, Alhamami MAM, Algadi H, Ansari ZA. Label-Free Myoglobin Biosensor Based on Pure and Copper-Doped Titanium Dioxide Nanomaterials. Biosensors. 2022; 12(12):1151. https://doi.org/10.3390/bios12121151
Chicago/Turabian StyleUmar, Ahmad, Mazharul Haque, Shafeeque G. Ansari, Hyung-Kee Seo, Ahmed A. Ibrahim, Mohsen A. M. Alhamami, Hassan Algadi, and Zubaida A. Ansari. 2022. "Label-Free Myoglobin Biosensor Based on Pure and Copper-Doped Titanium Dioxide Nanomaterials" Biosensors 12, no. 12: 1151. https://doi.org/10.3390/bios12121151