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Article

Hand-Powered Inertial Microfluidic Syringe-Tip Centrifuge

1
School of Mechanical Engineering, Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing 211189, China
2
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
*
Author to whom correspondence should be addressed.
Biosensors 2022, 12(1), 14; https://doi.org/10.3390/bios12010014
Submission received: 27 November 2021 / Revised: 27 December 2021 / Accepted: 28 December 2021 / Published: 29 December 2021
(This article belongs to the Special Issue Microfluidics for Biomedical Applications)

Abstract

Conventional sample preparation techniques require bulky and expensive instruments and are not compatible with next-generation point-of-care diagnostic testing. Here, we report a manually operated syringe-tip inertial microfluidic centrifuge (named i-centrifuge) for high-flow-rate (up to 16 mL/min) cell concentration and experimentally demonstrate its working mechanism and performance. Low-cost polymer films and double-sided tape were used through a rapid nonclean-room process of laser cutting and lamination bonding to construct the key components of the i-centrifuge, which consists of a syringe-tip flow stabilizer and a four-channel paralleled inertial microfluidic concentrator. The unstable liquid flow generated by the manual syringe was regulated and stabilized with the flow stabilizer to power inertial focusing in a four-channel paralleled concentrator. Finally, we successfully used our i-centrifuge for manually operated cell concentration. This i-centrifuge offers the advantages of low device cost, simple hand-powered operation, high-flow-rate processing, and portable device volume. Therefore, it holds potential as a low-cost, portable sample preparation tool for point-of-care diagnostic testing.
Keywords: inertial microfluidics; cell concentration; hand-powered; point-of-care diagnostic testing inertial microfluidics; cell concentration; hand-powered; point-of-care diagnostic testing

Share and Cite

MDPI and ACS Style

Xiang, N.; Ni, Z. Hand-Powered Inertial Microfluidic Syringe-Tip Centrifuge. Biosensors 2022, 12, 14. https://doi.org/10.3390/bios12010014

AMA Style

Xiang N, Ni Z. Hand-Powered Inertial Microfluidic Syringe-Tip Centrifuge. Biosensors. 2022; 12(1):14. https://doi.org/10.3390/bios12010014

Chicago/Turabian Style

Xiang, Nan, and Zhonghua Ni. 2022. "Hand-Powered Inertial Microfluidic Syringe-Tip Centrifuge" Biosensors 12, no. 1: 14. https://doi.org/10.3390/bios12010014

APA Style

Xiang, N., & Ni, Z. (2022). Hand-Powered Inertial Microfluidic Syringe-Tip Centrifuge. Biosensors, 12(1), 14. https://doi.org/10.3390/bios12010014

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