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Article

In Situ LSPR Sensing of Secreted Insulin in Organ-on-Chip

1
Biosensors for Bioengineering Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Baldiri I Reixac, 10-12, 08028 Barcelona, Spain
2
Plasmon Nano-Optics Group, ICFO-Institute for Photonics Sciences, The Barcelona Institute of Science and Technology, 08860 Barcelona, Spain
3
Diabetes and Obesity Research Laboratory, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain
4
Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas (CIBERDEM), 28029 Madrid, Spain
5
Nanophotonic Systems Laboratory, Department of Mechanical and Process Engineering, ETH Zurich, 8092 Zurich, Switzerland
6
ICREA-Institució Catalana de Recerca i Estudis Avançats, 08010 Barcelona, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Biosensors 2021, 11(5), 138; https://doi.org/10.3390/bios11050138
Received: 19 March 2021 / Revised: 17 April 2021 / Accepted: 25 April 2021 / Published: 28 April 2021
Organ-on-a-chip (OOC) devices offer new approaches for metabolic disease modeling and drug discovery by providing biologically relevant models of tissues and organs in vitro with a high degree of control over experimental variables for high-content screening applications. Yet, to fully exploit the potential of these platforms, there is a need to interface them with integrated non-labeled sensing modules, capable of monitoring, in situ, their biochemical response to external stimuli, such as stress or drugs. In order to meet this need, we aim here to develop an integrated technology based on coupling a localized surface plasmon resonance (LSPR) sensing module to an OOC device to monitor the insulin in situ secretion in pancreatic islets, a key physiological event that is usually perturbed in metabolic diseases such as type 2 diabetes (T2D). As a proof of concept, we developed a biomimetic islet-on-a-chip (IOC) device composed of mouse pancreatic islets hosted in a cellulose-based scaffold as a novel approach. The IOC was interfaced with a state-of-the-art on-chip LSPR sensing platform to monitor the in situ insulin secretion. The developed platform offers a powerful tool to enable the in situ response study of microtissues to external stimuli for applications such as a drug-screening platform for human models, bypassing animal testing. View Full-Text
Keywords: LSPR sensors; organ-on-a-chip; in situ insulin monitoring LSPR sensors; organ-on-a-chip; in situ insulin monitoring
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MDPI and ACS Style

Ortega, M.A.; Rodríguez-Comas, J.; Yavas, O.; Velasco-Mallorquí, F.; Balaguer-Trias, J.; Parra, V.; Novials, A.; Servitja, J.M.; Quidant, R.; Ramón-Azcón, J. In Situ LSPR Sensing of Secreted Insulin in Organ-on-Chip. Biosensors 2021, 11, 138. https://doi.org/10.3390/bios11050138

AMA Style

Ortega MA, Rodríguez-Comas J, Yavas O, Velasco-Mallorquí F, Balaguer-Trias J, Parra V, Novials A, Servitja JM, Quidant R, Ramón-Azcón J. In Situ LSPR Sensing of Secreted Insulin in Organ-on-Chip. Biosensors. 2021; 11(5):138. https://doi.org/10.3390/bios11050138

Chicago/Turabian Style

Ortega, María A., Júlia Rodríguez-Comas, Ozlem Yavas, Ferran Velasco-Mallorquí, Jordina Balaguer-Trias, Victor Parra, Anna Novials, Joan M. Servitja, Romain Quidant, and Javier Ramón-Azcón. 2021. "In Situ LSPR Sensing of Secreted Insulin in Organ-on-Chip" Biosensors 11, no. 5: 138. https://doi.org/10.3390/bios11050138

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