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Open AccessArticle

Electrochemical Immunosensor for Human IgE Using Ferrocene Self-Assembled Monolayers Modified ITO Electrode

1
Department of Chemistry, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea
2
Pioneering Research Center, LG Chem. Ltd., 30 Magokjungang 10-ro, Gangseo-gu, Seoul 07796, Korea
3
Human Convergence Technology Group, Korea Institute of Industrial Technology (KITECH), 143 Hanggaul-ro, Sangnok-gu, Ansan 15588, Korea
*
Authors to whom correspondence should be addressed.
Biosensors 2020, 10(4), 38; https://doi.org/10.3390/bios10040038
Received: 12 March 2020 / Revised: 10 April 2020 / Accepted: 12 April 2020 / Published: 14 April 2020
(This article belongs to the Special Issue Biosensors for Rapid Diagnostics)
The immunoglobulin E (IgE) level in serum is an important factor in the examination of allergy. Ferrocene (Fc)-modified self-assembled monolayers (SAMs) were placed on an indium tin oxide (ITO) electrode as a sensing layer for the detection of human IgE. The Fc moiety in the SAMs facilitated the electron transfer through the organic SAMs layer and electrocatalytic signal amplification. The electrochemical measurement was accomplished after the sandwich type immobilization of the receptor antibody, target human IgE, and enzyme conjugated secondary antibody. The enzyme product, p-aminophenol, was quantitatively analyzed by redox cycling via Fc. In addition, the electrochemical impedance spectroscopy (EIS) was investigated for the detection of IgE. The limit of detection (LOD), limit of quantification (LOQ), and dynamic range of the electrochemical sensor were 3 IU/mL, 10 IU/mL, and from 10 IU/mL to 100 IU/mL, respectively. View Full-Text
Keywords: human immunoglobulin E; indium tin oxide (ITO); electrocatalytic reaction; ferrocene; electrochemical impedance spectroscopy human immunoglobulin E; indium tin oxide (ITO); electrocatalytic reaction; ferrocene; electrochemical impedance spectroscopy
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Park, M.; Song, Y.; Kim, K.J.; Oh, S.J.; Ahn, J.K.; Park, H.; Shin, H.-B.; Kwon, S.J. Electrochemical Immunosensor for Human IgE Using Ferrocene Self-Assembled Monolayers Modified ITO Electrode. Biosensors 2020, 10, 38.

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