Colorimetric Diagnostic Capillary Enabled by Size Sieving in a Porous Hydrogel
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Assembly of the Diagnostic Capillary with a Porous Hydrogel
2.3. Preparation of Sandwiched Immunocomplex
2.3.1. Functionalization of Capture Particles
2.3.2. Functionalization of Probe Particles
2.3.3. Formation of Sandwiched Immunocomplex
2.4. Measurement Platform
2.5. Statistical Analysis
3. Results and Discussion
3.1. Formation of Porous Hydrogel
3.2. Sandwiched Immunocomplex Detection
3.3. Colorimetric Assessment for Direct Visualization
3.4. Quantitative Assessment with the Measurement Platform
3.5. Detection of Biomarker LCN 1 in Clinical Tear Samples
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Guzman, J.M.C.C.; Hsu, S.-M.; Chuang, H.-S. Colorimetric Diagnostic Capillary Enabled by Size Sieving in a Porous Hydrogel. Biosensors 2020, 10, 130. https://doi.org/10.3390/bios10100130
Guzman JMCC, Hsu S-M, Chuang H-S. Colorimetric Diagnostic Capillary Enabled by Size Sieving in a Porous Hydrogel. Biosensors. 2020; 10(10):130. https://doi.org/10.3390/bios10100130
Chicago/Turabian StyleGuzman, John Mello Camille C., Sheng-Min Hsu, and Han-Sheng Chuang. 2020. "Colorimetric Diagnostic Capillary Enabled by Size Sieving in a Porous Hydrogel" Biosensors 10, no. 10: 130. https://doi.org/10.3390/bios10100130