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Article

[email protected] Nanoparticles Functionalized with Oligonucleotides: A Prominent Tool for the Detection of the Methylated Reprimo Gene in Gastric Cancer by Dynamic Light Scattering

1
Laboratorio de Química Biológica, Instituto de Química, Pontificia Universidad Católica de Valparaíso, 2373223 Valparaíso, Chile
2
Departamento de Hematología y Oncología, Facultad de Medicina, Pontificia Universidad Católica de Chile, 8330032 Santiago, Chile
3
Advanced Center for Chronic Diseases (ACCDiS), Pontificia Universidad Católica de Chile, 8330034 Santiago, Chile
4
Departamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, 8380494 Independencia, Santiago, Chile
*
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(9), 1333; https://doi.org/10.3390/nano9091333
Received: 20 July 2019 / Revised: 7 September 2019 / Accepted: 12 September 2019 / Published: 18 September 2019
Reprimo (RPRM) is a tumor suppressor gene involved in the development of gastric cancer. Hypermethylation of the RPRM promoter region has been found in tumor tissue and plasma samples from patients with gastric cancer. These findings suggest that circulating methylated DNA of RPRM could be a candidate for a noninvasive detection of gastric cancer. We designed a nanosystem based on the functionalization of silica coated gold nanoparticles with oligonucleotides that recognize a specific DNA fragment of the RPRM promoter region. The functionality of the oligonucleotide on the surface of the nanoparticle was confirmed by polymerase chain reaction (PCR). The nanoparticles were incubated with a synthetic DNA fragment of methylated DNA of RPRM and changes in the size distribution after hybridization were evaluated by dynamic light scattering (DLS). A difference in the size distribution of nanoparticles hybridized with genomic DNA from the KATO III gastric cancer cell line was observed when was compared with DNA from the GES-1 normal cell line. These results showed that this nanosystem may be a useful tool for the specific and sensitive detection of methylated DNA of RPRM in patients at risk of developing gastric cancer. View Full-Text
Keywords: gold nanoparticles; methylated RPRM DNA; dynamic light scattering; gastric cancer; noninvasive detection gold nanoparticles; methylated RPRM DNA; dynamic light scattering; gastric cancer; noninvasive detection
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MDPI and ACS Style

Marchant, M.J.; Guzmán, L.; Corvalán, A.H.; Kogan, M.J. [email protected] Nanoparticles Functionalized with Oligonucleotides: A Prominent Tool for the Detection of the Methylated Reprimo Gene in Gastric Cancer by Dynamic Light Scattering. Nanomaterials 2019, 9, 1333. https://doi.org/10.3390/nano9091333

AMA Style

Marchant MJ, Guzmán L, Corvalán AH, Kogan MJ. [email protected] Nanoparticles Functionalized with Oligonucleotides: A Prominent Tool for the Detection of the Methylated Reprimo Gene in Gastric Cancer by Dynamic Light Scattering. Nanomaterials. 2019; 9(9):1333. https://doi.org/10.3390/nano9091333

Chicago/Turabian Style

Marchant, María J., Leda Guzmán, Alejandro H. Corvalán, and Marcelo J. Kogan 2019. "[email protected] Nanoparticles Functionalized with Oligonucleotides: A Prominent Tool for the Detection of the Methylated Reprimo Gene in Gastric Cancer by Dynamic Light Scattering" Nanomaterials 9, no. 9: 1333. https://doi.org/10.3390/nano9091333

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