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Open AccessArticle

Micronuclei Detection by Flow Cytometry as a High-Throughput Approach for the Genotoxicity Testing of Nanomaterials

1
Department of Genetics and Microbiology, Faculty of Biosciences, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
2
Consortium for Biomedical Research in Epidemiology and Public Health (CIBERESP), Carlos III Institute of Health, 28029 Madrid, Spain
3
Consortium for Biomedical Research on Rare Diseases (CIBERER), Carlos III Institute of Health, 28029 Madrid, Spain
*
Authors to whom correspondence should be addressed.
Nanomaterials 2019, 9(12), 1677; https://doi.org/10.3390/nano9121677
Received: 15 October 2019 / Revised: 19 November 2019 / Accepted: 22 November 2019 / Published: 24 November 2019
Thousands of nanomaterials (NMs)-containing products are currently under development or incorporated in the consumer market, despite our very limited understanding of their genotoxic potential. Taking into account that the toxicity and genotoxicity of NMs strongly depend on their physicochemical characteristics, many variables must be considered in the safety evaluation of each given NM. In this scenario, the challenge is to establish high-throughput methodologies able to generate rapid and robust genotoxicity data that can be used to critically assess and/or predict the biological effects associated with those NMs being under development or already present in the market. In this study, we have evaluated the advantages of using a flow cytometry-based approach testing micronucleus (MNs) induction (FCMN assay). In the frame of the EU NANoREG project, we have tested six different NMs—namely NM100 and NM101 (TiO2NPs), NM110 (ZnONPs), NM212 (CeO2NPs), NM300K (AgNPs) and NM401 (multi-walled carbon nanotubes (MWCNTs)). The obtained results confirm the ability of AgNPs and MWCNTs to induce MN in the human bronchial epithelial BEAS-2B cell line, whereas the other tested NMs retrieved non-significant increases in the MN frequency. Based on the alignment of the results with the data reported in the literature and the performance of the FCMN assay, we strongly recommend this assay as a reference method to systematically evaluate the potential genotoxicity of NMs.
Keywords: BEAS-2B cells; flow cytometry MN (FCMN) assay; TiO2NPs; ZnONPs; CeO2NPs; AgNPs; MWCNTs BEAS-2B cells; flow cytometry MN (FCMN) assay; TiO2NPs; ZnONPs; CeO2NPs; AgNPs; MWCNTs
MDPI and ACS Style

García-Rodríguez, A.; Kazantseva, L.; Vila, L.; Rubio, L.; Velázquez, A.; Ramírez, M.J.; Marcos, R.; Hernández, A. Micronuclei Detection by Flow Cytometry as a High-Throughput Approach for the Genotoxicity Testing of Nanomaterials. Nanomaterials 2019, 9, 1677.

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