Next Article in Journal
Supramolecular Modification of ABC Triblock Terpolymers in Confinement Assembly
Next Article in Special Issue
Micronuclei Detection by Flow Cytometry as a High-Throughput Approach for the Genotoxicity Testing of Nanomaterials
Previous Article in Journal
A Surface Mediated Supramolecular Chiral Phenomenon for Recognition of l- and d-Cysteine

Surface Charge-Dependent Cellular Uptake of Polystyrene Nanoparticles

Lab of Toxicology, Department of Medicinal Biotechnology, College of Health Sciences, Dong-A University, Busan 49315, Korea
EastChem, School of Chemistry, University of Edinburgh, David Brewster Road, Edinburgh EH9 3FJ, UK
Medicinal Chemistry, Cardiovascular, Renal and Metabolism, IMED Biotech Unit, AstraZeneca, Pepparedsleden 1, 431 50 Mölndal, Sweden
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Nanomaterials 2018, 8(12), 1028;
Received: 27 October 2018 / Revised: 4 December 2018 / Accepted: 8 December 2018 / Published: 10 December 2018
The evaluation of the role of physicochemical properties in the toxicity of nanoparticles is important for the understanding of toxicity mechanisms and for controlling the behavior of nanoparticles. The surface charge of nanoparticles is suggested as one of the key parameters which decide their biological impact. In this study, we synthesized fluorophore-conjugated polystyrene nanoparticles (F-PLNPs), with seven different types of surface functional groups that were all based on an identical core, to evaluate the role of surface charge in the cellular uptake of nanoparticles. Phagocytic differentiated THP-1 cells or non-phagocytic A549 cells were incubated with F-PLNP for 4 h, and their cellular uptake was quantified by fluorescence intensity and confocal microscopy. The amount of internalized F-PLNPs showed a good positive correlation with the zeta potential of F-PLNPs in both cell lines (Pearson’s r = 0.7021 and 0.7852 for zeta potential vs. cellular uptake in THP-1 cells and nonphagocytic A549 cells, respectively). This result implies that surface charge is the major parameter determining cellular uptake efficiency, although other factors such as aggregation/agglomeration, protein corona formation, and compositional elements can also influence the cellular uptake partly or indirectly. View Full-Text
Keywords: cellular uptake; fluorescence; surface charge; polystyrene nanoparticles; macrophage; epithelial cell cellular uptake; fluorescence; surface charge; polystyrene nanoparticles; macrophage; epithelial cell
Show Figures

Graphical abstract

MDPI and ACS Style

Jeon, S.; Clavadetscher, J.; Lee, D.-K.; Chankeshwara, S.V.; Bradley, M.; Cho, W.-S. Surface Charge-Dependent Cellular Uptake of Polystyrene Nanoparticles. Nanomaterials 2018, 8, 1028.

AMA Style

Jeon S, Clavadetscher J, Lee D-K, Chankeshwara SV, Bradley M, Cho W-S. Surface Charge-Dependent Cellular Uptake of Polystyrene Nanoparticles. Nanomaterials. 2018; 8(12):1028.

Chicago/Turabian Style

Jeon, Soyeon, Jessica Clavadetscher, Dong-Keun Lee, Sunay V. Chankeshwara, Mark Bradley, and Wan-Seob Cho. 2018. "Surface Charge-Dependent Cellular Uptake of Polystyrene Nanoparticles" Nanomaterials 8, no. 12: 1028.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop