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Open AccessArticle

Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection

1
Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (CBGP, UPM-INIA), Campus Montegancedo, Autopista M-40, km 38. Pozuelo de Alarcón, 28223 Madrid, Spain
2
Division of Hematopoietic Innovative Therapies, Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT) and Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBER-ER),28040 Madrid, Spain
3
Advanced Therapy Unit, Instituto de Investigación Sanitaria Fundación Jiménez Díaz (IIS-FJD/UAM), 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Nanomaterials 2019, 9(10), 1438; https://doi.org/10.3390/nano9101438
Received: 16 September 2019 / Revised: 2 October 2019 / Accepted: 3 October 2019 / Published: 10 October 2019
(This article belongs to the Special Issue Virus-Based Nanomaterials and Nanostructures)
Nanoparticles derived from the elongated flexuous capsids of Turnip mosaic virus (TuMV) have been shown to be efficient tools for antibody sensing with a very high sensitivity if adequately functionalized with the corresponding epitopes. Taking advantage of this possibility, TuMV virus-like particles (VLPs) have been genetically derivatized with a peptide from the chaperonin Hsp60, a protein described to be involved in inflammation processes and autoimmune diseases. Antibodies against the peptide have been previously shown to have a diagnostic value in at least one autoimmune disease, multiple sclerosis. The functionalized Hsp60-VLPs showed their significant increase in sensing potency when compared to monoclonal antibody detection of the peptide in a conventional immunoassay. Additionally, the developed Hsp60-VLPs allowed the detection of autoantibodies against the Hsp60 peptide in an in vivo mouse model of dextran sodium sulfate (DSS)-induced colitis. The detection of minute amounts of the autoantibodies allowed us to perform the analysis of their evolution during the progression of the disease. The anti-Hsp60 autoantibody levels in the sera of the inflamed mice went down during the induction phase of the disease. Increased levels of the anti-HSP60 autoantibodies were detected during the resolution phase of the disease. An extension of a previously proposed model for the involvement of Hsp60 in inflammatory processes is considered, incorporating a role for Hsp60 autoantibodies. This, and related models, can now be experimentally tested thanks to the autoantibody detection hypersensitivity provided by the functionalized VLPs.
Keywords: VNPs; Hsp60; IBD; autoantibody; inflammation; diagnosis VNPs; Hsp60; IBD; autoantibody; inflammation; diagnosis
MDPI and ACS Style

Yuste-Calvo, C.; López-Santalla, M.; Zurita, L.; Cruz-Fernández, C.F.; Sánchez, F.; Garín, M.I.; Ponz, F. Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection. Nanomaterials 2019, 9, 1438.

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