Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization
Abstract
:1. Introduction
2. Materials and Methods
2.1. Titanium Discs
2.2. Protein Adsorption
2.2.1. Surface Pre-Conditioning
2.2.2. Bradford Assay
2.3. Osteoblasts Adhesion Analysis
2.3.1. Cell Culture
2.3.2. Surface Conditioning
2.3.3. Adhesion Assay
2.3.4. Cell Morphology and Focal Adhesion Expression
2.3.5. Morphological Analysis of Osteoblast/Titanium Relationship
2.4. Statistical Analysis
3. Results
3.1. Serum Proteins Firmly Bind to Titanium Surfaces
3.2. Serum Proteins at Titanium Interface Increase the Strength of Cell Adhesion and Focal Adhesions Expression
3.3. Serum Proteins at Titanium Interface Did Not Affect Cell Morphology at SEM
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Parisi, L.; Toffoli, A.; Cutrera, M.; Bianchi, M.G.; Lumetti, S.; Bussolati, O.; Macaluso, G.M. Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization. Nanomaterials 2019, 9, 1407. https://doi.org/10.3390/nano9101407
Parisi L, Toffoli A, Cutrera M, Bianchi MG, Lumetti S, Bussolati O, Macaluso GM. Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization. Nanomaterials. 2019; 9(10):1407. https://doi.org/10.3390/nano9101407
Chicago/Turabian StyleParisi, Ludovica, Andrea Toffoli, Miriam Cutrera, Massimiliano G. Bianchi, Simone Lumetti, Ovidio Bussolati, and Guido M. Macaluso. 2019. "Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization" Nanomaterials 9, no. 10: 1407. https://doi.org/10.3390/nano9101407