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Open AccessArticle

Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization

1
Centro Universitario di Odontoiatria, Dipartimento di Medicina e Chirurgia, Università di Parma, 43126 Parma, Italy
2
Dipartimento di Medicina e Chirurgia, Università di Parma, 43126 Parma, Italy
3
Istituto dei Materiali per l’Elettronica ed il Magnetismo, Consiglio Nazionale delle Ricerche, Parco Area delle Scienze 37/A, 43124 Parma, Italy
*
Author to whom correspondence should be addressed.
The authors contributed equally to this work.
Nanomaterials 2019, 9(10), 1407; https://doi.org/10.3390/nano9101407
Received: 7 September 2019 / Revised: 23 September 2019 / Accepted: 30 September 2019 / Published: 2 October 2019
(This article belongs to the Special Issue Nanomaterials and Nanotechnology in Dentistry)
The host-material interface is a crucial relationship dictating the possibility of successful osseointegration in implant dentistry. The aim of the present study was to characterize the effects of plasma proteins pre-adsorption on the adhesion capacity of osteoblasts, which occurs immediately after implant insertion in vivo. After having pre-adsorbed human plasma proteins on a machined and microrough titanium surface, MC3T3-E1 osteoblasts adhesion was evaluated through crystal violet cell adhesion assay, immunofluorescence staining for cytoskeleton, focal adhesions and cell nuclei, and scanning electron microscopy. The pre-adsorbed protein layer markedly affected the adhesion rate of cells, as well as their morphology and the expression of focal contacts. Moreover, protein adsorption to the underlying titanium surface was found to be correlated to surface pre-wetting. Thus, the early adsorption of serum proteins to the interface of dental implants impacts cell adhesion in terms of strength and of focal adhesions expression. View Full-Text
Keywords: adsorption; focal adhesions; dental implant; osseointegration; osteoblasts; titanium adsorption; focal adhesions; dental implant; osseointegration; osteoblasts; titanium
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Parisi, L.; Toffoli, A.; Cutrera, M.; Bianchi, M.G.; Lumetti, S.; Bussolati, O.; Macaluso, G.M. Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization. Nanomaterials 2019, 9, 1407.

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