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Nanomaterials 2018, 8(8), 611; https://doi.org/10.3390/nano8080611

Markers of Oxidative Stress in the Exhaled Breath Condensate of Workers Handling Nanocomposites

1
Department of Occupational Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Na Bojisti 1, 128 00 Prague 2, Czech Republic
2
Institute of Chemical Process Fundamentals of the CAS, Rozvojová 1/135, 165 02 Prague 6, Czech Republic
3
Department of Machining and Assembly, Department of Engineering Technology, Department of Material Science, Faculty of Mechanical Engineering, Technical University in Liberec, Faculty of Mechanical Engineering, Studentská 1402/2, 461 17 Liberec, Czech Republic
4
Institute of Biophysics and Informatics, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Salmovská 1, 120 00 Prague 2, Czech Republic
5
Faculty of Physical Education and Sport, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, José Martího 31, 162 52 Prague 6, Czech Republic
6
Biocev, 1st Faculty of Medicine, Charles University, Prumyslova 595, 252 50 Vestec, Czech Republic
7
Department of Biomedical and Nutritional Sciences, Zuckerberg College of Health Sciences, Lowell, MA 01854, USA
*
Author to whom correspondence should be addressed.
Received: 17 July 2018 / Revised: 8 August 2018 / Accepted: 8 August 2018 / Published: 10 August 2018
(This article belongs to the Special Issue Toxicity and Ecotoxicity of Nanomaterials)
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Abstract

Researchers in nanocomposite processing may inhale a variety of chemical agents, including nanoparticles. This study investigated airway oxidative stress status in the exhaled breath condensate (EBC). Nineteen employees (42.4 ± 11.4 y/o), working in nanocomposites research for 18.0 ± 10.3 years were examined pre-shift and post-shift on a random workday, together with nineteen controls (45.5 ± 11.7 y/o). Panels of oxidative stress biomarkers derived from lipids, nucleic acids, and proteins were analyzed in the EBC. Aerosol exposures were monitored during three major nanoparticle generation operations: smelting and welding (workshop 1) and nanocomposite machining (workshop 2) using a suite of real-time and integrated instruments. Mass concentrations during these operations were 0.120, 1.840, and 0.804 mg/m3, respectively. Median particle number concentrations were 4.8 × 104, 1.3 × 105, and 5.4 × 105 particles/cm3, respectively. Nanoparticles accounted for 95, 40, and 61%, respectively, with prevailing Fe and Mn. All markers of nucleic acid and protein oxidation, malondialdehyde, and aldehydes C6–C13 were elevated, already in the pre-shift samples relative to controls in both workshops. Significant post-shift elevations were documented in lipid oxidation markers. Significant associations were found between working in nanocomposite synthesis and EBC biomarkers. More research is needed to understand the contribution of nanoparticles from nanocomposite processing in inducing oxidative stress, relative to other co-exposures generated during welding, smelting, and secondary oxidation processes, in these workshops. View Full-Text
Keywords: nanoparticles; workers; nanocomposites; inhalation; exhaled breath condensate; oxidative stress; occupational exposure nanoparticles; workers; nanocomposites; inhalation; exhaled breath condensate; oxidative stress; occupational exposure
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Pelclova, D.; Zdimal, V.; Schwarz, J.; Dvorackova, S.; Komarc, M.; Ondracek, J.; Kostejn, M.; Kacer, P.; Vlckova, S.; Fenclova, Z.; Popov, A.; Lischkova, L.; Zakharov, S.; Bello, D. Markers of Oxidative Stress in the Exhaled Breath Condensate of Workers Handling Nanocomposites. Nanomaterials 2018, 8, 611.

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