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Magnetic Polyion Complex Micelles for Cell Toxicity Induced by Radiofrequency Magnetic Field Hyperthermia

1
University Bordeaux, LCPO, UMR 5629, F-33600 Pessac, France
2
Department of Chemistry, University of Waterloo, Waterloo, ON N2L 3G1, Canada
3
CNRS, Laboratoire de Chimie des Polymères Organiques, UMR 5629, F-33600 Pessac, France
4
University of Liège, Mammalian Cell Culture Laboratory, GIGA-R, B-4000 Liège, Belgium
*
Authors to whom correspondence should be addressed.
Nanomaterials 2018, 8(12), 1014; https://doi.org/10.3390/nano8121014
Received: 11 November 2018 / Revised: 1 December 2018 / Accepted: 3 December 2018 / Published: 6 December 2018
(This article belongs to the Special Issue Magnetic Nanoparticles in Biological Applications)
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Abstract

Magnetic nanoparticles (MNPs) of magnetite (Fe3O4) were prepared using a polystyrene-graft-poly(2-vinylpyridine) copolymer (denoted G0PS-g-P2VP or G1) as template. These MNPs were subjected to self-assembly with a poly(acrylic acid)-block-poly(2-hydroxyethyl acrylate) double-hydrophilic block copolymer (DHBC), PAA-b-PHEA, to form water-dispersible magnetic polyion complex (MPIC) micelles. Large Fe3O4 crystallites were visualized by transmission electron microscopy (TEM) and magnetic suspensions of MPIC micelles exhibited improved colloidal stability in aqueous environments over a wide pH and ionic strength range. Biological cells incubated for 48 h with MPIC micelles at the highest concentration (1250 µg of Fe3O4 per mL) had a cell viability of 91%, as compared with 51% when incubated with bare (unprotected) MNPs. Cell internalization, visualized by confocal laser scanning microscopy (CLSM) and TEM, exhibited strong dependence on the MPIC micelle concentration and incubation time, as also evidenced by fluorescence-activated cell sorting (FACS). The usefulness of MPIC micelles for cellular radiofrequency magnetic field hyperthermia (MFH) was also confirmed, as the MPIC micelles showed a dual dose-dependent effect (concentration and duration of magnetic field exposure) on the viability of L929 mouse fibroblasts and U87 human glioblastoma epithelial cells. View Full-Text
Keywords: magnetic polyion complex micelle; arborescent polymer; double-hydrophilic block copolymer; cytotoxicity; cell internalization; magnetic field hyperthermia magnetic polyion complex micelle; arborescent polymer; double-hydrophilic block copolymer; cytotoxicity; cell internalization; magnetic field hyperthermia
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Nguyen, V.T.A.; De Pauw-Gillet, M.-C.; Gauthier, M.; Sandre, O. Magnetic Polyion Complex Micelles for Cell Toxicity Induced by Radiofrequency Magnetic Field Hyperthermia. Nanomaterials 2018, 8, 1014.

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