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Influence of Sterilization and Preservation Procedures on the Integrity of Serum Protein-Coated Magnetic Nanoparticles

1
Institute of Biomedical Engineering and Informatics (BMTI), Technische Universität Ilmenau, Gustav-Kirchhoff-Strasse 2, D-98693 Ilmenau, Germany
2
Department of Nano Biophotonics, Leibniz Institute of Photonic Technology (IPHT), A.-Einstein-Strasse 9, D-07745 Jena, Germany
3
Department Hematology and Oncology, Jena University Hospital, Am Klinikum 1, D-07747 Jena, Germany
*
Author to whom correspondence should be addressed.
Nanomaterials 2017, 7(12), 453; https://doi.org/10.3390/nano7120453
Received: 30 October 2017 / Revised: 8 December 2017 / Accepted: 12 December 2017 / Published: 15 December 2017
(This article belongs to the Special Issue Magnetic Nanoparticles in Biological Applications)
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Abstract

Protein-coated magnetic nanoparticles are promising candidates for various medical applications. Prior to their application into a biological system, one has to guarantee that the particle dispersions are free from pathogens or any other microbiologic contamination. Furthermore, to find entrance into clinical routine, the nanoparticle dispersions have to be storable for several months. In this study, we tested several procedures for sterilization and preservation of nanoparticle containing liquids on their influence on the integrity of the protein coating on the surface of these particles. For this, samples were treated by freezing, autoclaving, lyophilization, and ultraviolet (UV) irradiation, and characterized by means of dynamic light scattering, determination of surface potential, and gel electrophoresis afterwards. We found that the UV sterilization followed by lyophilization under the addition of polyethylene glycol are the most promising procedures for the preparation of sterilized long-term durable protein-coated magnetic nanoparticles. Ongoing work is focused on the optimization of used protocols for UV sterilization and lyophilization for further improvement of the storage time. View Full-Text
Keywords: magnetic nanoparticles; superparamagnetic iron oxide nanoparticles; protein corona; UV sterilization magnetic nanoparticles; superparamagnetic iron oxide nanoparticles; protein corona; UV sterilization
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Dutz, S.; Wojahn, S.; Gräfe, C.; Weidner, A.; Clement, J.H. Influence of Sterilization and Preservation Procedures on the Integrity of Serum Protein-Coated Magnetic Nanoparticles. Nanomaterials 2017, 7, 453.

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