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Nanomaterials
Volume 12
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10.3390/nano12030503
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Article

Silk ProteinsEnriched Nanocomposite Hydrogels Based on Modified MMT Clay and Poly(2-hydroxyethyl methacrylate-co-2-acrylamido-2-methylpropane Sulfonic Acid) Display Favorable Properties for Soft Tissue Engineering

by
Mirela Violeta Șerban
1,†,
Simona-Rebeca Nazarie (Ignat)
1,†,
Sorina Dinescu
1,2,*,
Ionuț-Cristian Radu
3,
Cătălin Zaharia
3,
Elena-Alexandra Istrătoiu
3,
Eugenia Tănasă
3,
Hildegard Herman
4,
Sami Gharbia
4,
Cornel Baltă
4,
Anca Hermenean
4,* and
Marieta Costache
1,2
1
Department of Biochemistry and Molecular Biology, University of Bucharest, 050663 Bucharest, Romania
2
The Research Institute of the University of Bucharest (ICUB), University of Bucharest, 050663 Bucharest, Romania
3
Advanced Polymer Materials Group, University Politehnica of Bucharest, 011061 Bucharest, Romania
4
“Aurel Ardelean” Institute of Life Sciences, ”Vasile Goldiș” Western University of Arad, 310025 Arad, Romania
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Nanomaterials 2022, 12(3), 503; https://doi.org/10.3390/nano12030503
Submission received: 24 December 2021 / Revised: 25 January 2022 / Accepted: 27 January 2022 / Published: 31 January 2022
(This article belongs to the Special Issue Biocompatibility of Nanomaterials in Medical Applications)
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Abstract

Due to their remarkable structures and properties, three-dimensional hydrogels and nanostructured clay particles have been extensively studied and have shown a high potential for tissue engineering as solutions for tissue defects. In this study, four types of 2-hydroxyethyl methacrylate/2-acrylamido-2-methylpropane sulfonic acid/montmorillonite (HEMA/AMPSA/MMT) hydrogels enriched with sericin, and fibroin were prepared and studied in the context of regenerative medicine for soft tissue regenerative medicine. Our aim was to obtain crosslinked hydrogel structures using modified montmorillonite clay as a crosslinking agent. In order to improve the in vitro and in vivo biocompatibility, silk proteins were further incorporated within the hydrogel matrix. Fourier transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR) were performed to prove the chemical structures of the modified MMT and nanocomposite hydrogels. Swelling and rheological measurements showed the good elastic behavior of the hydrogels due to this unique network structure in which modified MMT acts as a crosslinking agent. Hydrogel biocompatibility was assessed by MTT, LDH and LIVE/DEAD assays. The hydrogels were evaluated for their potential to support adipogenesis in vitro and human stem cells isolated from adipose tissue were seeded in them and induced to differentiate. The progress was assessed by evaluation of expression of adipogenic markers (ppar-γ2, perilipin) evaluated by qPCR. The potential of the materials to support tissue regeneration was further evaluated on animal models in vivo. All materials proved to be biocompatible, with better results on the 95% HEMA 5% AMPSA enriched with sericin and fibroin material. This composition promoted a better development of adipogenesis compared to the other compositions studied, due the addition of sericin and fibroin. The results were confirmed in vivo as well, with a better progress of soft tissue regeneration after implantation in mice. Therefore, hydrogel 95% HEMA 5% AMPSA enriched with sericin as well as fibroin showed the best results that recommend it for future soft tissue engineering application.
Keywords: modified MMT; nanocomposite hydrogels; sericin; fibroin; adipogenesis modified MMT; nanocomposite hydrogels; sericin; fibroin; adipogenesis

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MDPI and ACS Style

Șerban, M.V.; Nazarie, S.-R.; Dinescu, S.; Radu, I.-C.; Zaharia, C.; Istrătoiu, E.-A.; Tănasă, E.; Herman, H.; Gharbia, S.; Baltă, C.; et al. Silk ProteinsEnriched Nanocomposite Hydrogels Based on Modified MMT Clay and Poly(2-hydroxyethyl methacrylate-co-2-acrylamido-2-methylpropane Sulfonic Acid) Display Favorable Properties for Soft Tissue Engineering. Nanomaterials 2022, 12, 503. https://doi.org/10.3390/nano12030503

AMA Style

Șerban MV, Nazarie S-R, Dinescu S, Radu I-C, Zaharia C, Istrătoiu E-A, Tănasă E, Herman H, Gharbia S, Baltă C, et al. Silk ProteinsEnriched Nanocomposite Hydrogels Based on Modified MMT Clay and Poly(2-hydroxyethyl methacrylate-co-2-acrylamido-2-methylpropane Sulfonic Acid) Display Favorable Properties for Soft Tissue Engineering. Nanomaterials. 2022; 12(3):503. https://doi.org/10.3390/nano12030503

Chicago/Turabian Style

Șerban, Mirela Violeta, Simona-Rebeca Nazarie (Ignat), Sorina Dinescu, Ionuț-Cristian Radu, Cătălin Zaharia, Elena-Alexandra Istrătoiu, Eugenia Tănasă, Hildegard Herman, Sami Gharbia, Cornel Baltă, and et al. 2022. "Silk ProteinsEnriched Nanocomposite Hydrogels Based on Modified MMT Clay and Poly(2-hydroxyethyl methacrylate-co-2-acrylamido-2-methylpropane Sulfonic Acid) Display Favorable Properties for Soft Tissue Engineering" Nanomaterials 12, no. 3: 503. https://doi.org/10.3390/nano12030503

APA Style

Șerban, M. V., Nazarie, S.-R., Dinescu, S., Radu, I.-C., Zaharia, C., Istrătoiu, E.-A., Tănasă, E., Herman, H., Gharbia, S., Baltă, C., Hermenean, A., & Costache, M. (2022). Silk ProteinsEnriched Nanocomposite Hydrogels Based on Modified MMT Clay and Poly(2-hydroxyethyl methacrylate-co-2-acrylamido-2-methylpropane Sulfonic Acid) Display Favorable Properties for Soft Tissue Engineering. Nanomaterials, 12(3), 503. https://doi.org/10.3390/nano12030503

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