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Nanomaterials
Volume 12
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10.3390/nano12010004
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Article

DNAzyme-Amplified Electrochemical Biosensor Coupled with pH Meter for Ca2+ Determination at Variable pH Environments

by
Hui Wang
1,
Fan Zhang
1,2,
Yue Wang
1,3,
Fangquan Shi
4,
Qingyao Luo
1,
Shanshan Zheng
1,
Junhong Chen
5,
Dingzhen Dai
5,
Liang Yang
1,*,
Xiangfang Tang
1,* and
Benhai Xiong
1,*
1
State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2
College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
3
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China
4
Animal Husbandry and Veterinary Station of Xihe County, Longnan 742100, China
5
Department of Animal Science and Technology, Jinling Institute of Technology, Nanjing 211169, China
*
Authors to whom correspondence should be addressed.
Nanomaterials 2022, 12(1), 4; https://doi.org/10.3390/nano12010004
Submission received: 8 November 2021 / Revised: 8 December 2021 / Accepted: 14 December 2021 / Published: 21 December 2021
(This article belongs to the Topic Advances and Applications of Carbon Nanotubes)
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Abstract

For more than 50% of multiparous cows, it is difficult to adapt to the sudden increase in calcium demand for milk production, which is highly likely to cause hypocalcemia. An electrochemical biosensor is a portable and efficient method to sense Ca2+ concentrations, but biomaterial is easily affected by the pH of the analyte solution. Here, an electrochemical biosensor was fabricated using a glassy carbon electrode (GCE) and single-walled carbon nanotube (SWNT), which amplified the impedance signal by changing the structure and length of the DNAzyme. Aiming at the interference of the pH, the electrochemical biosensor (GCE/SWNT/DNAzyme) was coupled with a pH meter to form an electrochemical device. It was used to collect data at different Ca2+ concentrations and pH values, and then was processed using different mathematical models, of which GPR showed higher detecting accuracy. After optimizing the detecting parameters, the electrochemical device could determine the Ca2+ concentration ranging from 5 μM to 25 mM, with a detection limit of 4.2 μM at pH values ranging from 4.0 to 7.5. Finally, the electrochemical device was used to determine the Ca2+ concentrations in different blood and milk samples, which can overcome the influence of the pH.
Keywords: electrochemical sensor; single-walled carbon nanotube; DNAzyme; dairy cow; hypocalcemia electrochemical sensor; single-walled carbon nanotube; DNAzyme; dairy cow; hypocalcemia
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MDPI and ACS Style

Wang, H.; Zhang, F.; Wang, Y.; Shi, F.; Luo, Q.; Zheng, S.; Chen, J.; Dai, D.; Yang, L.; Tang, X.; et al. DNAzyme-Amplified Electrochemical Biosensor Coupled with pH Meter for Ca2+ Determination at Variable pH Environments. Nanomaterials 2022, 12, 4. https://doi.org/10.3390/nano12010004

AMA Style

Wang H, Zhang F, Wang Y, Shi F, Luo Q, Zheng S, Chen J, Dai D, Yang L, Tang X, et al. DNAzyme-Amplified Electrochemical Biosensor Coupled with pH Meter for Ca2+ Determination at Variable pH Environments. Nanomaterials. 2022; 12(1):4. https://doi.org/10.3390/nano12010004

Chicago/Turabian Style

Wang, Hui, Fan Zhang, Yue Wang, Fangquan Shi, Qingyao Luo, Shanshan Zheng, Junhong Chen, Dingzhen Dai, Liang Yang, Xiangfang Tang, and et al. 2022. "DNAzyme-Amplified Electrochemical Biosensor Coupled with pH Meter for Ca2+ Determination at Variable pH Environments" Nanomaterials 12, no. 1: 4. https://doi.org/10.3390/nano12010004

APA Style

Wang, H., Zhang, F., Wang, Y., Shi, F., Luo, Q., Zheng, S., Chen, J., Dai, D., Yang, L., Tang, X., & Xiong, B. (2022). DNAzyme-Amplified Electrochemical Biosensor Coupled with pH Meter for Ca2+ Determination at Variable pH Environments. Nanomaterials, 12(1), 4. https://doi.org/10.3390/nano12010004

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