Next Article in Journal
Acid-Responsive Adamantane-Cored Amphiphilic Block Polymers as Platforms for Drug Delivery
Previous Article in Journal
Multipolar Plasmonic Resonances of Aluminum Nanoantenna Tuned by Graphene
Previous Article in Special Issue
Direct and Label-Free Monitoring of Albumin in 2D Fatty Liver Disease Model Using Plasmonic Nanogratings
Article

Novel Regeneration Approach for Creating Reusable FO-SPR Probes with NTA Surface Chemistry

1
Biosensors Group, Department of Biosystems, KU Leuven, Willem de Croylaan 42, 3001 Leuven, Belgium
2
Department of Synthetic Biology and Immunology, National Institute of Chemistry, 1000 Ljubljana, Slovenia
*
Authors to whom correspondence should be addressed.
Nanomaterials 2021, 11(1), 186; https://doi.org/10.3390/nano11010186
Received: 14 December 2020 / Revised: 7 January 2021 / Accepted: 11 January 2021 / Published: 13 January 2021
(This article belongs to the Special Issue Nanomechanical and Optical Biosensors)
To date, surface plasmon resonance (SPR) biosensors have been exploited in numerous different contexts while continuously pushing boundaries in terms of improved sensitivity, specificity, portability and reusability. The latter has attracted attention as a viable alternative to disposable biosensors, also offering prospects for rapid screening of biomolecules or biomolecular interactions. In this context here, we developed an approach to successfully regenerate a fiber-optic (FO)-SPR surface when utilizing cobalt (II)-nitrilotriacetic acid (NTA) surface chemistry. To achieve this, we tested multiple regeneration conditions that can disrupt the NTA chelate on a surface fully saturated with His6-tagged antibody fragments (scFv-33H1F7) over ten regeneration cycles. The best surface regeneration was obtained when combining 100 mM EDTA, 500 mM imidazole and 0.5% SDS at pH 8.0 for 1 min with shaking at 150 rpm followed by washing with 0.5 M NaOH for 3 min. The true versatility of the established approach was proven by regenerating the NTA surface for ten cycles with three other model system bioreceptors, different in their size and structure: His6-tagged SARS-CoV-2 spike fragment (receptor binding domain, RBD), a red fluorescent protein (RFP) and protein origami carrying 4 RFPs (Tet12SN-RRRR). Enabling the removal of His6-tagged bioreceptors from NTA surfaces in a fast and cost-effective manner can have broad applications, spanning from the development of biosensors and various biopharmaceutical analyses to the synthesis of novel biomaterials. View Full-Text
Keywords: NTA chemistry; surface regeneration; fiber-optic-surface plasmon resonance (FO-SPR); biosensor; His-tag; antibody fragment; SARS-CoV-2 receptor-binding domain (RBD); red fluorescent protein (RFP); protein origami (Tet12SN-RRRR) NTA chemistry; surface regeneration; fiber-optic-surface plasmon resonance (FO-SPR); biosensor; His-tag; antibody fragment; SARS-CoV-2 receptor-binding domain (RBD); red fluorescent protein (RFP); protein origami (Tet12SN-RRRR)
Show Figures

Figure 1

MDPI and ACS Style

Qu, J.-H.; Leirs, K.; Escudero, R.; Strmšek, Ž.; Jerala, R.; Spasic, D.; Lammertyn, J. Novel Regeneration Approach for Creating Reusable FO-SPR Probes with NTA Surface Chemistry. Nanomaterials 2021, 11, 186. https://doi.org/10.3390/nano11010186

AMA Style

Qu J-H, Leirs K, Escudero R, Strmšek Ž, Jerala R, Spasic D, Lammertyn J. Novel Regeneration Approach for Creating Reusable FO-SPR Probes with NTA Surface Chemistry. Nanomaterials. 2021; 11(1):186. https://doi.org/10.3390/nano11010186

Chicago/Turabian Style

Qu, Jia-Huan, Karen Leirs, Remei Escudero, Žiga Strmšek, Roman Jerala, Dragana Spasic, and Jeroen Lammertyn. 2021. "Novel Regeneration Approach for Creating Reusable FO-SPR Probes with NTA Surface Chemistry" Nanomaterials 11, no. 1: 186. https://doi.org/10.3390/nano11010186

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop