Next Article in Journal
Luminescent Ionogels with Excellent Transparency, High Mechanical Strength, and High Conductivity
Next Article in Special Issue
Novel Regeneration Approach for Creating Reusable FO-SPR Probes with NTA Surface Chemistry
Previous Article in Journal
Detection of Single Molecules Using Stochastic Resonance of Bistable Oligomers
Previous Article in Special Issue
Portable Real-Time Detection of Pb(II) Using a CMOS MEMS-Based Nanomechanical Sensing Array Modified with PEDOT:PSS
Article

Direct and Label-Free Monitoring of Albumin in 2D Fatty Liver Disease Model Using Plasmonic Nanogratings

1
Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology, Baldiri I Reixac, 10-12, 08028 Barcelona, Spain
2
ICREA-Institució Catalana de Recerca i Estudis Avançats, 08010 Barcelona, Spain
*
Author to whom correspondence should be addressed.
Nanomaterials 2020, 10(12), 2520; https://doi.org/10.3390/nano10122520
Received: 18 November 2020 / Revised: 11 December 2020 / Accepted: 11 December 2020 / Published: 15 December 2020
(This article belongs to the Special Issue Nanomechanical and Optical Biosensors)
Non-alcoholic fatty liver (NAFLD) is a metabolic disorder related to a chronic lipid accumulation within the hepatocytes. This disease is the most common liver disorder worldwide, and it is estimated that it is present in up to 25% of the world’s population. However, the real prevalence of this disease and the associated disorders is unknown mainly because reliable and applicable diagnostic tools are lacking. It is known that the level of albumin, a pleiotropic protein synthesized by hepatocytes, is correlated with the correct function of the liver. The development of a complementary tool that allows direct, sensitive, and label-free monitoring of albumin secretion in hepatocyte cell culture can provide insight into NAFLD’s mechanism and drug action. With this aim, we have developed a simple integrated plasmonic biosensor based on gold nanogratings from periodic nanostructures present in commercial Blu-ray optical discs. This sensor allows the direct and label-free monitoring of albumin in a 2D fatty liver disease model under flow conditions using a highly-specific polyclonal antibody. This technology avoids both the amplification and blocking steps showing a limit of detection within pM range (≈0.26 ng/mL). Thanks to this technology, we identified the optimal fetal bovine serum (FBS) concentration to maximize the cells’ lipid accumulation. Moreover, we discovered that the hepatocytes increased the amount of albumin secreted on the third day from the lipids challenge. These data demonstrate the ability of hepatocytes to respond to the lipid stimulation releasing more albumin. Further investigation is needed to unveil the biological significance of that cell behavior. View Full-Text
Keywords: 2D fatty liver in vitro model; Blu-Ray disc; plasmonic nanomaterials; Label-Free Biosensing 2D fatty liver in vitro model; Blu-Ray disc; plasmonic nanomaterials; Label-Free Biosensing
Show Figures

Graphical abstract

MDPI and ACS Style

Lopez-Muñoz, G.A.; Ortega, M.A.; Ferret-Miñana, A.; De Chiara, F.; Ramón-Azcón, J. Direct and Label-Free Monitoring of Albumin in 2D Fatty Liver Disease Model Using Plasmonic Nanogratings. Nanomaterials 2020, 10, 2520. https://doi.org/10.3390/nano10122520

AMA Style

Lopez-Muñoz GA, Ortega MA, Ferret-Miñana A, De Chiara F, Ramón-Azcón J. Direct and Label-Free Monitoring of Albumin in 2D Fatty Liver Disease Model Using Plasmonic Nanogratings. Nanomaterials. 2020; 10(12):2520. https://doi.org/10.3390/nano10122520

Chicago/Turabian Style

Lopez-Muñoz, Gerardo A., Maria A. Ortega, Ainhoa Ferret-Miñana, Francesco De Chiara, and Javier Ramón-Azcón. 2020. "Direct and Label-Free Monitoring of Albumin in 2D Fatty Liver Disease Model Using Plasmonic Nanogratings" Nanomaterials 10, no. 12: 2520. https://doi.org/10.3390/nano10122520

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop