Effect of Flavonoid-Coated Gold Nanoparticles on Bacterial Colonization in Mice Organs
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains
2.2. Flavonoid Extraction
2.3. Green Synthesis of FAuNPs
2.4. Physical Characterizations of FAuNPs
2.5. In-Vitro Stability of FAuNPs
2.6. Antibacterial Susceptibility of Flavonoids by Qualitative Method
2.7. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of CF and FAuNPs Against E. faecalis
2.8. Hemolysis Assay
2.9. Colonization of E. faecalis in BALB/c Mice
2.10. In Vivo Antibacterial Activity of CF and FAuNPs
2.11. Statistical Analysis
3. Results and Discussion
3.1. Antimicrobial Activity of Flavonoids
3.2. Synthesis and Physical Characterizations of FAuNPs
3.2.1. Impact of Physico-Chemical Parameters on FAuNPs Synthesis and Its Stability
3.2.2. FTIR Analysis of CF and FAuNPs
3.2.3. XRD Analysis of FAuNPs
3.2.4. STEM Analysis of FAuNPs
3.2.5. EDS Analysis (EDAX) of FAuNPs
3.3. In Vitro Antibacterial Activity of FAuNPs
3.4. Hemolysis Assay
3.5. In Vivo Anticolonizing Potential of CF and FAuNPs in Infectious Mice Model
4. Conclusions and Perspectives
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
B. lyceum: | Berberis lycium |
C.E: | Crude extract |
C.F: | Conjugated flavonoids |
E. faecalis: | Enterococcus faecalis |
EDS: | Energy-dispersive X-ray spectroscopy |
FAuNPs: | Flavonoid gold nanoparticles |
F.F: | Free flavonoids |
FTIR: | Fourier-transform infrared spectroscopy |
HAuCl4: | Tetrachloroauric acid |
IV: | Intravenous |
MDR: | Multi-drug resistance |
NPs: | Nanoparticles |
RBCs: | Red blood cells |
STEM: | Scanning transmission electron microscopy |
UTI: | Urinary tract infection |
UV-Vis: | Ultraviolet-visible |
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Riaz, S.; Fatima Rana, N.; Hussain, I.; Tanweer, T.; Nawaz, A.; Menaa, F.; Janjua, H.A.; Alam, T.; Batool, A.; Naeem, A.; et al. Effect of Flavonoid-Coated Gold Nanoparticles on Bacterial Colonization in Mice Organs. Nanomaterials 2020, 10, 1769. https://doi.org/10.3390/nano10091769
Riaz S, Fatima Rana N, Hussain I, Tanweer T, Nawaz A, Menaa F, Janjua HA, Alam T, Batool A, Naeem A, et al. Effect of Flavonoid-Coated Gold Nanoparticles on Bacterial Colonization in Mice Organs. Nanomaterials. 2020; 10(9):1769. https://doi.org/10.3390/nano10091769
Chicago/Turabian StyleRiaz, Sundus, Nosheen Fatima Rana, Irshad Hussain, Tahreem Tanweer, Afrah Nawaz, Farid Menaa, Hussnain A. Janjua, Tahseen Alam, Amna Batool, Ayesha Naeem, and et al. 2020. "Effect of Flavonoid-Coated Gold Nanoparticles on Bacterial Colonization in Mice Organs" Nanomaterials 10, no. 9: 1769. https://doi.org/10.3390/nano10091769