Next Article in Journal
Engineering Core-Shell Structures of Magnetic Ferrite Nanoparticles for High Hyperthermia Performance
Previous Article in Journal
Enhanced Photocatalytic Properties of PET Filaments Coated with Ag-N Co-Doped TiO2 Nanoparticles Sensitized with Disperse Blue Dyes
Previous Article in Special Issue
Incorporation of Lippia citriodora Microwave Extract into Total-Green Biogelatin-Phospholipid Vesicles to Improve Its Antioxidant Activity
Open AccessArticle

Freeze-Dried Softisan® 649-Based Lipid Nanoparticles for Enhanced Skin Delivery of Cyclosporine A

1
LAQV, REQUIMTE, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
2
UCIBIO, REQUIMTE, MedTech, Departamento de Ciências do Medicamento, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
3
Serviço de Dermatologia do Centro Hospitalar e Universitário do Porto, Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Rua D. Manuel II, s/n, 4099-001 Porto, Portugal
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Nanomaterials 2020, 10(5), 986; https://doi.org/10.3390/nano10050986
Received: 27 April 2020 / Revised: 18 May 2020 / Accepted: 19 May 2020 / Published: 21 May 2020
(This article belongs to the Special Issue Nanocarriers and Targeted Drug Delivery)
Inflammatory skin diseases, including psoriasis and atopic dermatitis, affect around one quarter to one third of the world population. Systemic cyclosporine A, an immunosuppressant agent, is included in the current therapeutic armamentarium of these diseases. Despite being highly effective, it is associated with several side effects, and its topical administration is limited by its high molecular weight and poor water solubility. To overcome these limitations, cyclosporine A was incorporated into solid lipid nanoparticles obtained from Softisan® 649, a commonly used cosmetic ingredient, aiming to develop a vehicle for application to the skin. The nanoparticles presented sizes of around 200 nm, low polydispersity, negative surface charge, and stability when stored for 8 weeks at room temperature or 4 °C. An effective incorporation of 88% of cyclosporine A within the nanoparticles was observed, without affecting its morphology. After the freeze-drying process, the Softisan® 649-based nanoparticles formed an oleogel. Skin permeation studies using pig ear as a model revealed low permeation of the applied cyclosporine A in the freeze-dried form of the nanoparticles in relation to free drug and the freshly prepared nanoparticles. About 1.0 mg of cyclosporine A was delivered to the skin with reduced transdermal permeation. These results confirm local delivery of cyclosporine A, indicating its promising topical administration. View Full-Text
Keywords: cellular uptake; Franz-cell permeation; keratinocytes; pig ear skin; pseudoplastic properties; solid lipid nanoparticles cellular uptake; Franz-cell permeation; keratinocytes; pig ear skin; pseudoplastic properties; solid lipid nanoparticles
Show Figures

Graphical abstract

MDPI and ACS Style

Silva, M.I.; Barbosa, A.I.; Costa Lima, S.A.; Costa, P.; Torres, T.; Reis, S. Freeze-Dried Softisan® 649-Based Lipid Nanoparticles for Enhanced Skin Delivery of Cyclosporine A. Nanomaterials 2020, 10, 986.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop