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Open AccessArticle

Influences of Nanoparticles Characteristics on the Cellular Responses: The Example of Iron Oxide and Macrophages

Grenoble Alpes University, CNRS, CEA, Laboratory of Chemistry and Biology of Metals, BIG-LCBM, 38000 Grenoble, France
Grenoble Alpes University, CNRS, CEA, INAC, SyMMES, RSRM, 38000 Grenoble, France
Institut de Biologie Structurale (IBS), University Grenoble Alpes, CEA, CNRS, 38044 Grenoble, France
Grenoble Alpes University, CEA-Grenoble, LITEN, DTNM, L2N, 17 rue des Martyrs, CEDEX 09, 38054 Grenoble, France
Integrated Structural Biology Grenoble (ISBG) CNRS, CEA, Université Grenoble Alpes, EMBL, 71 Avenue des Martyrs, 38042 Grenoble, France
Authors to whom correspondence should be addressed.
Deceased person (21 November 2018).
Nanomaterials 2020, 10(2), 266;
Received: 30 December 2019 / Revised: 24 January 2020 / Accepted: 1 February 2020 / Published: 5 February 2020
Iron oxide nanoparticles/microparticles are widely present in a variety of environments, e.g., as a byproduct of steel and iron degradation, as, for example, in railway brakes (e.g., metro station) or in welding fumes. As all particulate material, these metallic nanoparticles are taken up by macrophages, a cell type playing a key role in the innate immune response, including pathogen removal phagocytosis, secretion of free radical species such as nitric oxide or by controlling inflammation via cytokine release. In this paper, we evaluated how macrophages functions were altered by two iron based particles of different size (100 nm and 20 nm). We showed that at high, but subtoxic concentrations (1 mg/mL, large nanoparticles induced stronger perturbations in macrophages functions such as phagocytic capacity (tested with fluorescent latex microspheres) and the ability to respond to bacterial endotoxin lipopolysaccharide stimulus (LPS) in secreting nitric oxide and pro-cytokines (e.g., Interleukin-6 (IL-6) and Tumor Necrosis Factor (TNF)). These stronger effects may correlate with an observed stronger uptake of iron for the larger nanoparticles.
Keywords: macrophage; iron oxide; nanoparticle macrophage; iron oxide; nanoparticle
MDPI and ACS Style

Dalzon, B.; Torres, A.; Reymond, S.; Gallet, B.; Saint-Antonin, F.; Collin-Faure, V.; Moriscot, C.; Fenel, D.; Schoehn, G.; Aude-Garcia, C.; Rabilloud, T. Influences of Nanoparticles Characteristics on the Cellular Responses: The Example of Iron Oxide and Macrophages. Nanomaterials 2020, 10, 266.

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