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Open AccessFeature PaperArticle

Topology Challenge for the Assessment of Living Cell Deposits with Shear Bulk Acoustic Biosensor

1
FEMTO-ST Institute, CNRS UMR-6174, University Bourgogne Franche-Comté, 25000 Besançon, France
2
Institute for Micro and Sensor Systems, Otto-von-Guericke-University Magdeburg, 39106 Magdeburg, Germany
3
Geneva Platelet Group, Faculty of Medicine, Geneva University, 1205 Geneva, Switzerland
4
Haemostasis Unit, Department of Medical Specialities, University Hospital Geneva (HUG), 1205 Geneva, Switzerland
*
Authors to whom correspondence should be addressed.
Nanomaterials 2020, 10(10), 2079; https://doi.org/10.3390/nano10102079
Received: 28 September 2020 / Revised: 15 October 2020 / Accepted: 16 October 2020 / Published: 21 October 2020
(This article belongs to the Special Issue Advanced Studies in Nano-BioAnalytical Physico-Chemistry)
Shear bulk acoustic type of resonant biosensors, such as the quartz crystal microbalance (QCM), give access to label-free in-liquid analysis of surface interactions. The general understanding of the sensing principles was inherited from past developments in biofilms measurements and applied to cells while keeping the same basic assumptions. Thus, the biosensor readouts are still quite often described using ‘mass’ related terminology. This contribution aims to show that assessment of cell deposits with acoustic biosensors requires a deep understanding of the sensor transduction mechanism. More specifically, the cell deposits should be considered as a structured viscoelastic load and the sensor response depends on both material and topological parameters of the deposits. This shifts the paradigm of acoustic biosensor away from the classical mass loading perspective. As a proof of the concept, we recorded QCM frequency shifts caused by blood platelet deposits on a collagen surface under different rheological conditions and observed the final deposit shape with atomic force microscopy (AFM). The results vividly demonstrate that the frequency shift is highly impacted by the platelet topology on the bio-interface. We support our findings with numerical simulations of viscoelastic unstructured and structured loads in liquid. Both experimental and theoretical studies underline the complexity behind the frequency shift interpretation when acoustic biosensing is used with cell deposits. View Full-Text
Keywords: acoustic biosensor; QCM; platelet; cells surface topology acoustic biosensor; QCM; platelet; cells surface topology
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MDPI and ACS Style

Oseev, A.; Mukhin, N.; Elie-Caille, C.; Boireau, W.; Lucklum, R.; Lecompte, T.; Remy-Martin, F.; Manceau, J.-F.; Chollet, F.; Leblois, T. Topology Challenge for the Assessment of Living Cell Deposits with Shear Bulk Acoustic Biosensor. Nanomaterials 2020, 10, 2079. https://doi.org/10.3390/nano10102079

AMA Style

Oseev A, Mukhin N, Elie-Caille C, Boireau W, Lucklum R, Lecompte T, Remy-Martin F, Manceau J-F, Chollet F, Leblois T. Topology Challenge for the Assessment of Living Cell Deposits with Shear Bulk Acoustic Biosensor. Nanomaterials. 2020; 10(10):2079. https://doi.org/10.3390/nano10102079

Chicago/Turabian Style

Oseev, Aleksandr; Mukhin, Nikolay; Elie-Caille, Céline; Boireau, Wilfrid; Lucklum, Ralf; Lecompte, Thomas; Remy-Martin, Fabien; Manceau, Jean-François; Chollet, Franck; Leblois, Thérèse. 2020. "Topology Challenge for the Assessment of Living Cell Deposits with Shear Bulk Acoustic Biosensor" Nanomaterials 10, no. 10: 2079. https://doi.org/10.3390/nano10102079

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