Next Article in Journal
Pre-Clinical Cell-Based Therapy for Limbal Stem Cell Deficiency
Next Article in Special Issue
How Sensitive Is the Elasticity of Hydroxyapatite-Nanoparticle-Reinforced Chitosan Composite to Changes in Particle Concentration and Crystallization Temperature?
Previous Article in Journal
Grafts for Ridge Preservation
Previous Article in Special Issue
Development of Thermosensitive Hydrogels of Chitosan, Sodium and Magnesium Glycerophosphate for Bone Regeneration Applications
Open AccessArticle

Injectable Amorphous Chitin-Agarose Composite Hydrogels for Biomedical Applications

Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Kochi 682041, India
Author to whom correspondence should be addressed.
Academic Editor: Kazuo Azuma
J. Funct. Biomater. 2015, 6(3), 849-862;
Received: 30 July 2015 / Revised: 13 August 2015 / Accepted: 17 August 2015 / Published: 25 August 2015
(This article belongs to the Special Issue Biomedical Applications of Chitin and Chitosan)
Injectable hydrogels are gaining popularity as tissue engineering constructs because of their ease of handling and minimal invasive delivery. Making hydrogels from natural polymers helps to overcome biocompatibility issues. Here, we have developed an Amorphous Chitin (ACh)-Agarose (Agr) composite hydrogel using a simpletechnique. Rheological studies, such as viscoelastic behavior (elastic modulus, viscous modulus, yield stress, and consistency), inversion test, and injectability test, were carried out for different ACh-Agr concentrations. The composite gel, having a concentration of 1.5% ACh and 0.25% Agr, showed good elastic modulus (17.3 kPa), yield stress (3.8 kPa), no flow under gravity, injectability, and temperature stability within the physiological range. Based on these studies, the optimum concentration for injectability was found to be 1.5% ACh and 0.25% Agr. This optimized concentration was used for further studies and characterized using FT-IR and SEM. FT-IR studies confirmed the presence of ACh and Agr in the composite gel. SEM results showed that the lyophilized composite gel had good porosity and mesh like networks. The cytocompatibility of the composite gel was studied using human mesenchymal stem cells (hMSCs). The composite gels showed good cell viability.These results indicated that this injectable composite gel can be used for biomedical applications. View Full-Text
Keywords: injectable hydrogel; amorphous chitin; agarose; biomedical applications injectable hydrogel; amorphous chitin; agarose; biomedical applications
Show Figures

Figure 1

MDPI and ACS Style

Priya, M.V.; Kumar, R.A.; Sivashanmugam, A.; Nair, S.V.; Jayakumar, R. Injectable Amorphous Chitin-Agarose Composite Hydrogels for Biomedical Applications. J. Funct. Biomater. 2015, 6, 849-862.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

Only visits after 24 November 2015 are recorded.
Back to TopTop