Artemisia argyi-Mediated Synthesis of Monodisperse Silver Nanoparticles as Components of Bioactive Nanofibrous Dressings with Dual Antibacterial and Regenerative Functions

Wang, Jiale; Guan, Jiawei; Ma, Xingyu; Zhao, Dongyang; Han, Yongqiang; Guo, Dongdong; Bai, Jialin; Guo, Zisheng; Zhang, Xiaojun

doi:10.3390/jfb16070236

This is an early access version, the complete PDF, HTML, and XML versions will be available soon.

Open AccessArticle

Artemisia argyi-Mediated Synthesis of Monodisperse Silver Nanoparticles as Components of Bioactive Nanofibrous Dressings with Dual Antibacterial and Regenerative Functions

by

Jiale Wang

^1,†

,

Jiawei Guan

^1,†

,

Xingyu Ma

¹,

Dongyang Zhao

¹

,

Yongqiang Han

¹

,

Dongdong Guo

²,

Jialin Bai

¹,

Zisheng Guo

^2,*

and

Xiaojun Zhang

^1,*

¹

School of Medicine, Northwest University, Xi’an 710069, China

²

Key Laboratory of Resource Biology and Biotechnology Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest University, Xi’an 710069, China

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

J. Funct. Biomater. 2025, 16(7), 236; https://doi.org/10.3390/jfb16070236 (registering DOI)

Submission received: 29 May 2025 / Revised: 23 June 2025 / Accepted: 26 June 2025 / Published: 27 June 2025

(This article belongs to the Section Biomaterials for Tissue Engineering and Regenerative Medicine)

Download Versions Notes

Abstract

The effective healing of chronic wounds requires balancing antimicrobial activity with tissue regeneration. In this study, we developed a novel, eco-friendly synthesis method using Artemisia argyi extract to produce silver nanoparticles (AgNPs), addressing toxicity concerns associated with conventional chemical synthesis methods. Through optimization of multiple synthesis parameters, monodisperse spherical AgNPs with an average diameter of 6.76 ± 0.27 nm were successfully obtained. Plant-derived compounds from Artemisia argyi extract acted as efficient mediators for both reduction and stabilization, yielding nanoparticles with high crystallinity. The synthesized AgNPs exhibited potent antibacterial activity against both Gram-negative and Gram-positive bacteria, with minimum inhibitory concentrations of 8 μg/mL against Escherichia coli and 32 μg/mL against Staphylococcus aureus, while maintaining high biocompatibility with L929 fibroblasts at concentrations ≤ 8 μg/mL. When integrated into polylactic acid/collagen type I (PLA/Col1) nanofibrous matrices, the optimized 0.03% AgNPs/PLA/Col1 dressing significantly accelerated wound healing in a diabetic rat model, achieving 94.62 ± 2.64% wound closure by day 14 compared to 65.81 ± 1.80% observed in untreated controls. Histological analyses revealed a dual-functional mechanism wherein controlled silver ion release provided sustained antibacterial protection, while concurrently promoting tissue regeneration characterized by enhanced collagen deposition, reduced inflammation, and increased neovascularization. This innovative approach effectively addresses critical challenges in diabetic wound care by providing simultaneous antimicrobial and regenerative functions within a single biomaterial platform.

Keywords: silver nanoparticles; Artemisia argyi; biomaterial; antimicrobial; wound healing

Share and Cite

MDPI and ACS Style

Wang, J.; Guan, J.; Ma, X.; Zhao, D.; Han, Y.; Guo, D.; Bai, J.; Guo, Z.; Zhang, X. Artemisia argyi-Mediated Synthesis of Monodisperse Silver Nanoparticles as Components of Bioactive Nanofibrous Dressings with Dual Antibacterial and Regenerative Functions. J. Funct. Biomater. 2025, 16, 236. https://doi.org/10.3390/jfb16070236

AMA Style

Wang J, Guan J, Ma X, Zhao D, Han Y, Guo D, Bai J, Guo Z, Zhang X. Artemisia argyi-Mediated Synthesis of Monodisperse Silver Nanoparticles as Components of Bioactive Nanofibrous Dressings with Dual Antibacterial and Regenerative Functions. Journal of Functional Biomaterials. 2025; 16(7):236. https://doi.org/10.3390/jfb16070236

Chicago/Turabian Style

Wang, Jiale, Jiawei Guan, Xingyu Ma, Dongyang Zhao, Yongqiang Han, Dongdong Guo, Jialin Bai, Zisheng Guo, and Xiaojun Zhang. 2025. "Artemisia argyi-Mediated Synthesis of Monodisperse Silver Nanoparticles as Components of Bioactive Nanofibrous Dressings with Dual Antibacterial and Regenerative Functions" Journal of Functional Biomaterials 16, no. 7: 236. https://doi.org/10.3390/jfb16070236

APA Style

Wang, J., Guan, J., Ma, X., Zhao, D., Han, Y., Guo, D., Bai, J., Guo, Z., & Zhang, X. (2025). Artemisia argyi-Mediated Synthesis of Monodisperse Silver Nanoparticles as Components of Bioactive Nanofibrous Dressings with Dual Antibacterial and Regenerative Functions. Journal of Functional Biomaterials, 16(7), 236. https://doi.org/10.3390/jfb16070236

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Artemisia argyi-Mediated Synthesis of Monodisperse Silver Nanoparticles as Components of Bioactive Nanofibrous Dressings with Dual Antibacterial and Regenerative Functions

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI