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Article

Preliminary Results on Heparin-Modified Double-Layered PCL and PLA-Based Scaffolds for Tissue Engineering of Small Blood Vessels

Department of Biomaterials and Composites, Faculty of Materials Science and Ceramics, AGH University of Science and Technology, 30-059 Kraków, Poland
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Academic Editor: R. Jayakumar
J. Funct. Biomater. 2022, 13(1), 11; https://doi.org/10.3390/jfb13010011
Received: 15 December 2021 / Revised: 18 January 2022 / Accepted: 24 January 2022 / Published: 27 January 2022
(This article belongs to the Special Issue Bioinspired Materials for Medical and Biotechnological Applications)
Low-diameter blood vessels are challenging to replace with more traditional synthetic vascular grafts. Therefore, the obvious choice is to try to regenerate small veins and arteries through tissue-engineering approaches. However, the layered structure of native vessels and blood compatibility issues make this a very challenging task. The aim of this study is to create double-layered tubular scaffolds with enhanced anticoagulant properties for the tissue engineering of small blood vessels. The scaffolds were made of a polycaprolactone-based porous outer layer and a polylactide-based electrospun inner layer modified with heparin. The combination of thermally induced phase separation and electrospinning resulted in asymmetric scaffolds with improved mechanical properties. The release assay confirmed that heparin is released from the scaffolds. Additionally, anticoagulant activity was shown through APTT (activated partial thromboplastin time) assay. Interestingly, the endothelial cell culture test revealed that after 14 days of culture, HAECs (human aortic endothelial cell lines) tended to organize in chain-like structures, typical for early stages of vascular formation. In the longer culture, HAEC viability was higher for the heparin-modified scaffolds. The proposed scaffold design and composition have great potential for application in tissue engineering of small blood vessels. View Full-Text
Keywords: vascular scaffold; polylactide; polycaprolactone; heparin release; blood vessels tissue engineering vascular scaffold; polylactide; polycaprolactone; heparin release; blood vessels tissue engineering
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MDPI and ACS Style

Domalik-Pyzik, P.; Morawska-Chochół, A. Preliminary Results on Heparin-Modified Double-Layered PCL and PLA-Based Scaffolds for Tissue Engineering of Small Blood Vessels. J. Funct. Biomater. 2022, 13, 11. https://doi.org/10.3390/jfb13010011

AMA Style

Domalik-Pyzik P, Morawska-Chochół A. Preliminary Results on Heparin-Modified Double-Layered PCL and PLA-Based Scaffolds for Tissue Engineering of Small Blood Vessels. Journal of Functional Biomaterials. 2022; 13(1):11. https://doi.org/10.3390/jfb13010011

Chicago/Turabian Style

Domalik-Pyzik, Patrycja, and Anna Morawska-Chochół. 2022. "Preliminary Results on Heparin-Modified Double-Layered PCL and PLA-Based Scaffolds for Tissue Engineering of Small Blood Vessels" Journal of Functional Biomaterials 13, no. 1: 11. https://doi.org/10.3390/jfb13010011

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