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Volume 15
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10.3390/agriculture15030241
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Article

Isolation of Acetic Acid-Producing Bacterial Strains and Utilization as Microbial Inoculants in Sorghum Silages

by
Guilherme Medeiros Leite
1,
Edson Mauro Santos
1,
Juliana Silva de Oliveira
1,
Danillo Marte Pereira
2,*,
Celso José Bruno de Oliveira
1,
Jorge Vinicius Fernandes Lima Cavalcanti
3,
Vanessa Maria Rodrigues de Lima
1,
João Paulo Vieira de Melo Fernandes de Lima
1,
Paloma Gabriela Batista Gomes
1,
Ricardo Loiola Edvan
4,
Rafael de Souza Miranda
3,
Daniele de Jesus Ferreira
2,
Fagton de Mattos Negrão
5 and
Anderson de Moura Zanine
2
1
Department of Animal Science, Federal University of Paraíba, Areia 58397-000, PB, Brazil
2
Department of Animal Science, Federal University of Maranhão, Chapadinha 65500-000, MA, Brazil
3
Department of Chemical Engineering, Federal University of Pernambuco, Recife 50670-901, PE, Brazil
4
Department of Animal Science, Federal University of Piauí, Teresina 64049-550, PI, Brazil
5
Department of Animal Science, Federal Institute of Education, Science, and Technology of Rondônia, Colorado do Oeste 76993-000, RO, Brazil
*
Author to whom correspondence should be addressed.
Agriculture 2025, 15(3), 241; https://doi.org/10.3390/agriculture15030241
Submission received: 1 December 2024 / Revised: 11 January 2025 / Accepted: 18 January 2025 / Published: 23 January 2025
(This article belongs to the Special Issue Current Challenges in Microbiology and Chemistry of Animal Feed)
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Abstract

This study aimed to isolate, characterize, and identify acetic acid-producing lactic acid bacteria from fresh sorghum plants and silage, and to evaluate the effect of the isolates as microbial inoculants on taxonomic diversity and silage fermentation quality. For the first experimental stage, eight experimental silos were prepared, and the fresh sorghum plant cv. BRS Ponta Negra (Sorghum bicolor (L.) Moench.) was sampled to characterize and identify the bacteria. Five strains were chosen to be inoculated in the second experimental stage, in a 7 × 2 factorial design, with seven treatments and two opening times, in four replications. Four types of species were identified, with Lactiplantibacillus plantarum predominating at 72.73%. There was an interaction effect between treatments and opening times on effluent losses, gas losses, the population of lactic acid bacteria, yeasts, and lactic acid content. The aerobic stability treatments that stood out were Lactiplantibacillus plantarum (GML 66) and Weissella cibaria, which showed 71.75 and 68.87 h of stability. The use of Lactiplantibacillus plantarum (GML 66) as a microbial inoculant in sorghum silage increased the dry matter content, reduced effluent losses, and improved dry matter recovery. It also reduced the yeast population in the silage, promoting greater aerobic stability in the silage.
Keywords: acetic acid; aerobic stability; forage conservation; Lactiplantibacillus plantarum; fermentation quality acetic acid; aerobic stability; forage conservation; Lactiplantibacillus plantarum; fermentation quality

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MDPI and ACS Style

Leite, G.M.; Santos, E.M.; de Oliveira, J.S.; Pereira, D.M.; de Oliveira, C.J.B.; Cavalcanti, J.V.F.L.; de Lima, V.M.R.; de Lima, J.P.V.d.M.F.; Gomes, P.G.B.; Edvan, R.L.; et al. Isolation of Acetic Acid-Producing Bacterial Strains and Utilization as Microbial Inoculants in Sorghum Silages. Agriculture 2025, 15, 241. https://doi.org/10.3390/agriculture15030241

AMA Style

Leite GM, Santos EM, de Oliveira JS, Pereira DM, de Oliveira CJB, Cavalcanti JVFL, de Lima VMR, de Lima JPVdMF, Gomes PGB, Edvan RL, et al. Isolation of Acetic Acid-Producing Bacterial Strains and Utilization as Microbial Inoculants in Sorghum Silages. Agriculture. 2025; 15(3):241. https://doi.org/10.3390/agriculture15030241

Chicago/Turabian Style

Leite, Guilherme Medeiros, Edson Mauro Santos, Juliana Silva de Oliveira, Danillo Marte Pereira, Celso José Bruno de Oliveira, Jorge Vinicius Fernandes Lima Cavalcanti, Vanessa Maria Rodrigues de Lima, João Paulo Vieira de Melo Fernandes de Lima, Paloma Gabriela Batista Gomes, Ricardo Loiola Edvan, and et al. 2025. "Isolation of Acetic Acid-Producing Bacterial Strains and Utilization as Microbial Inoculants in Sorghum Silages" Agriculture 15, no. 3: 241. https://doi.org/10.3390/agriculture15030241

APA Style

Leite, G. M., Santos, E. M., de Oliveira, J. S., Pereira, D. M., de Oliveira, C. J. B., Cavalcanti, J. V. F. L., de Lima, V. M. R., de Lima, J. P. V. d. M. F., Gomes, P. G. B., Edvan, R. L., Miranda, R. d. S., Ferreira, D. d. J., Negrão, F. d. M., & Zanine, A. d. M. (2025). Isolation of Acetic Acid-Producing Bacterial Strains and Utilization as Microbial Inoculants in Sorghum Silages. Agriculture, 15(3), 241. https://doi.org/10.3390/agriculture15030241

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