Selection and Phenotyping for Drought Tolerance in Somatic Hybrids between Solanum tuberosum and Solanum bulbocastanum That Show Resistance to Late Blight, by Using a Semi-Automated Plant Phenotyping Platform
Abstract
:1. Introduction
2. Materials and Methods
2.1. In Vitro Drought Stress Selection
2.2. Statistical Analysis
2.3. Biomass Accumulation of Somatic Hybrids under Drought Stress Ex Vitro
2.4. Evaluation of Drought Stress Effect on Photosynthesis
3. Results
3.1. In Vitro Drought Stress Selection
3.2. Proline Content
3.3. Ex Vitro Plant Development in Somatic Hybrids under Drought Stress
3.4. Tuber Yield
3.5. Effect of Drought Stress on Photosynthesis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Treatment | Group 1 Genotypes | Group 2 Genotypes | Group 3 Genotypes | Group 4 Genotypes |
---|---|---|---|---|
Media supplemented with 5% PEG | SH 99/2 | SH 82/4/68 | Solanum tuberosum cv. Delikat | - |
SH 84/5 | SH 94/5 | Solanum bulbocastanum | ||
SH 95/1 | BC2 95/1/4/11 | BC1 94/5/5 | ||
BC183/9/64 | BC1 82/4/4 | |||
SH 83/9 | BC1 82/4/38 | |||
SH 82/4 | ||||
BC2 82/4/68/22 | ||||
BC1 83/9/63 | ||||
BC1 83/9/3 | ||||
Media supplemented with 15% PEG | - | SH 94/5 | SH 83/9 | BC1 83/9/27 |
BC1 94/5/5 | BC1 83/9/63 | BC1 83/9/64 | ||
BC2 95/1/4/11 | Solanum tuberosum | BC2 95/1/4/59 | ||
BC2 82/4/68/22 | Solanum bulbocastanum | |||
BC1 95/1/3 | SH 95/1 | |||
SH 99/2 | BC1 95/1/7 | |||
SH 84/5 | ||||
SH 82/4 |
Genotype | Generation | Yield Changes % |
---|---|---|
Solanum tuberosum cv. Delikat | parent | 53 ↓ |
Solanum bulbocastanum | parent | No tubers |
SH 83/9 | Hybrid | 83.63 ↓ |
BC1 83/9/3 | BC1 | 72.81 ↓ |
SH 99/2 | Hybrid | 96.75 ↓ |
SH 82/4 | SH | 85.27 ↓ |
BC1 82/4/68 | BC1 | 21.38 ↓ |
BC2 82/4/68/22 | BC2 | 100 ↓ |
SH 95/1 | Hybrid | 90.72 ↓ |
BC1 95/1/7 | BC1 | 48.58 ↑ |
BC2 95/1/4/11 | BC2 | 71.66 ↓ |
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Dénes, T.-É.; Molnár, I.; Vass, I.Z.; Vass, I.; Rákosy-Tican, E. Selection and Phenotyping for Drought Tolerance in Somatic Hybrids between Solanum tuberosum and Solanum bulbocastanum That Show Resistance to Late Blight, by Using a Semi-Automated Plant Phenotyping Platform. Agriculture 2024, 14, 48. https://doi.org/10.3390/agriculture14010048
Dénes T-É, Molnár I, Vass IZ, Vass I, Rákosy-Tican E. Selection and Phenotyping for Drought Tolerance in Somatic Hybrids between Solanum tuberosum and Solanum bulbocastanum That Show Resistance to Late Blight, by Using a Semi-Automated Plant Phenotyping Platform. Agriculture. 2024; 14(1):48. https://doi.org/10.3390/agriculture14010048
Chicago/Turabian StyleDénes, Tünde-Éva, Imola Molnár, István Zoltán Vass, Imre Vass, and Elena Rákosy-Tican. 2024. "Selection and Phenotyping for Drought Tolerance in Somatic Hybrids between Solanum tuberosum and Solanum bulbocastanum That Show Resistance to Late Blight, by Using a Semi-Automated Plant Phenotyping Platform" Agriculture 14, no. 1: 48. https://doi.org/10.3390/agriculture14010048