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Stepwise-Selected Bacillus amyloliquefaciens and B. subtilis Strains from Composted Aromatic Plant Waste Able to Control Soil-Borne Diseases

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Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria, Centro di ricerca Orticoltura e Florovivaismo, Via Cavalleggeri 25, 84098 Pontecagnano Faiano (SA), Italy
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Dipartimento di Farmacia, Università degli Studi di Salerno, Via Giovanni Paolo II 132, 84084 Fisciano (SA), Italy
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Author to whom correspondence should be addressed.
Agriculture 2020, 10(2), 30; https://doi.org/10.3390/agriculture10020030
Received: 21 December 2019 / Revised: 11 January 2020 / Accepted: 21 January 2020 / Published: 23 January 2020
(This article belongs to the Special Issue Composting and Organic Soil Amendments)
In the present study, 133 bacterial isolates from 11 composted aromatic plant wastes were selected for their ability to inhibit the mycelial growth of the soil-borne phytopathogenic fungi Sclerotinia minor and Rhizoctonia solani. Successively, a subset of 35 from them were further characterized for their ability to control, in vivo, rocket damping-off caused by the two fungi. Moreover, the isolates were characterized for morphology of the colonies, Gram reaction, siderophore production, P-solubilization and for the presence of antimicrobial lipopeptide genes in the genome. The screening for the in vitro antagonisms showed a mycelial growth reduction ranging between 31.7% and 56.1% for R. solani and 34.4% and 59.4% for S. minor. All the isolates were not able to produce siderophores and some of them were able to solubilize P. The isolates contained two or more of the five lipoproteins coding genes investigated in this study. The most promising isolates were identified at species level by 16S-rRNA partial gene sequence analysis and were grouped in two main clusters related to Bacillus subtilis and Bacillus amyloliquefaciens reference strains. Results indicated that Bacillus isolates from compost are good candidates for application in the biocontrol of cultivated plants. View Full-Text
Keywords: antagonists; biological control; compost; lipopedtide genes; sustainable agriculture antagonists; biological control; compost; lipopedtide genes; sustainable agriculture
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Zaccardelli, M.; Sorrentino, R.; Caputo, M.; Scotti, R.; De Falco, E.; Pane, C. Stepwise-Selected Bacillus amyloliquefaciens and B. subtilis Strains from Composted Aromatic Plant Waste Able to Control Soil-Borne Diseases. Agriculture 2020, 10, 30.

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