Assessing Viral Shedding and Infectivity of Asymptomatic or Mildly Symptomatic Patients with COVID-19 in a Later Phase
Abstract
:1. Introduction
2. Methods
2.1. Patients and Study Settings
2.2. Definitions
2.3. Nucleic Acid Extraction and RT-PCR for SARS-CoV-2
2.4. SARS-CoV-2 Cell Culture
3. Results
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Variables | Total (n = 48) | Symptomatic Group (n = 37) | Asymptomatic Group (n = 11) | p Value |
---|---|---|---|---|
Age, years | 32.62 ± 14.59 | 31.84 ± 13.74 | 35.27 ± 17.62 | 0.499 |
Male, yes | 14 (29.2) | 12 (32.4) | 2 (18.2) | 0.361 |
Smoker | 5 (10.4) | 5 (13.5) | 0 (0.0) | 0.198 |
Comorbidity | ||||
Hypertension | 4 (8.3) | 2 (5.4) | 2 (18.2) | 0.178 |
Allergy | 4 (8.3) | 2 (5.4) | 2 (18.2) | 0.178 |
Malignancy | 2 (4.2) | 2 (5.4) | 0 (0.0) | 0.431 |
Diabetes | 1 (2.1) | 1 (2.7) | 0 (0.0) | 0.582 |
Asthma | 1 (2.1) | 1 (2.7) | 0 (0.0) | 0.582 |
Symptoms | ||||
Myalgia | 12 (25.0) | 12 (32.4) | 0 (0.0) | - |
Fever | 11 (22.9) | 11 (29.7) | 0 (0.0) | - |
Chill | 9 (18.8) | 9 (24.3) | 0 (0.0) | - |
Headache | 9 (18.3) | 9 (24.3) | 0 (0.0) | - |
Anosmia | 8 (16.7) | 8 (21.6) | 0 (0.0) | - |
Ageusia | 7 (14.6) | 7 (18.9) | 0 (0.0) | - |
Rhinorrhea | 7 (14.6) | 7 (18.9) | 0 (0.0) | - |
Nasal stuffiness | 7 (14.6) | 7 (18.9) | 0 (0.0) | - |
Fatigue | 7 (14.6) | 7 (18.9) | 0 (0.0) | - |
Cough | 6 (12.5) | 6 (16.2) | 0 (0.0) | - |
Sputum | 6 (12.5) | 6 (16.2) | 0 (0.0) | - |
Sore throat | 4 (8.3) | 4 (10.8) | 0 (0.0) | - |
Dizziness | 3 (6.3) | 3 (8.1) | 0 (0.0) | - |
Diarrhea | 3(6.3) | 3 (8.1) | 0 (0.0) | |
Chest pain | 1 (2.1) | 1 (2.7) | 0 (0.0) | - |
Vomiting | 1 (2.1) | 1 (2.7) | 0 (0.0) | - |
Symptomatic Group (n = 37) | Asymptomatic Group (n = 11) | p Value | |
---|---|---|---|
Period from diagnosis to culture | |||
Mean | 30.78 ± 5.85 | 29.09 ± 5.26 | 0.394 |
Median | 31 (20–40) | 28 (21–41) | |
Mean of Ct value on the day of culture | |||
E gene | 30.55 ± 2.96 | 27.60 ± 0.47 | - |
RdRP gene | 33.93 ± 3.43 | 31.56 ± 3.07 | - |
N gene | 34.98 ± 2.89 | 33.94 ± 3.65 | - |
Cases with rebound of Ct value | 11 (29.7) | 6 (54.5) | 0.131 |
Items | Age | Sex | Comorbidity | Symptoms | Rebound | Ct Value | |
---|---|---|---|---|---|---|---|
NPS | Saliva | ||||||
Patient1 | 51 | M | N | Y | N | 36.6 | 31.1 |
Patient2 | 21 | M | Allergy | Y | N | 38.9 | 37.0 |
Patient3 | 23 | F | N | Y | N | 35.1 | 31.4 |
Patient4 | 25 | F | N | Y | N | 32.2 | 35.2 |
Patient5 | 31 | F | N | Y | N | 33.2 | 36.9 |
Patient6 | 54 | F | N | N | N | 34.5 | 35.1 |
Patient7 | 27 | F | N | Y | N | 25.4 | 29.3 |
Patient8 | 33 | M | N | Y | Y | 0.0 | 35.7 |
Average of Ct value | 33.7 | 33.96 |
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Sohn, Y.; Jeong, S.J.; Chung, W.S.; Hyun, J.H.; Baek, Y.J.; Cho, Y.; Kim, J.H.; Ahn, J.Y.; Choi, J.Y.; Yeom, J.-S. Assessing Viral Shedding and Infectivity of Asymptomatic or Mildly Symptomatic Patients with COVID-19 in a Later Phase. J. Clin. Med. 2020, 9, 2924. https://doi.org/10.3390/jcm9092924
Sohn Y, Jeong SJ, Chung WS, Hyun JH, Baek YJ, Cho Y, Kim JH, Ahn JY, Choi JY, Yeom J-S. Assessing Viral Shedding and Infectivity of Asymptomatic or Mildly Symptomatic Patients with COVID-19 in a Later Phase. Journal of Clinical Medicine. 2020; 9(9):2924. https://doi.org/10.3390/jcm9092924
Chicago/Turabian StyleSohn, Yujin, Su Jin Jeong, Won Suk Chung, Jong Hoon Hyun, Yae Jee Baek, Yunsuk Cho, Jung Ho Kim, Jin Young Ahn, Jun Yong Choi, and Joon-Sup Yeom. 2020. "Assessing Viral Shedding and Infectivity of Asymptomatic or Mildly Symptomatic Patients with COVID-19 in a Later Phase" Journal of Clinical Medicine 9, no. 9: 2924. https://doi.org/10.3390/jcm9092924