Effects of Time-Interval since Blood Draw and of Anticoagulation on Platelet Testing (Count, Indices and Impedance Aggregometry): A Systematic Study with Blood from Healthy Volunteers
Abstract
:1. Introduction
2. Materials and Methods
Statistical Analysis
3. Results
3.1. Platelet Count
3.2. Platelet Indices
3.3. Platelet Function—MEA
3.4. Platelet Clumps
4. Discussion
4.1. Platelet Clumps
4.2. Platelet Count
4.3. Platelet Indices
4.4. Platelet Function
4.5. Limitations
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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5 min | 30 min (vs. 5 min) | 60 min (vs. 5 min) | 120 min (vs. 5 min) | 180 min (vs. 5 min) | |||||
---|---|---|---|---|---|---|---|---|---|
EDTA | 0 | 0.4 (3.8%) (0.3; 0.5) | *** | 0.6 (6.2%) (0.5; 0.7) | *** | 0.7 (7.2%) (0.6; 0.8) | *** | 0.8 (7.9%) (0.7; 0.9) | *** |
Hirudin | 0 | 0.6 (6.2%) (0.5; 0.7) | *** | 0.7 (7.6%) (0.6; 0.8) | *** | 0.7 (8.0%) (0.6; 0.9) | *** | 0.9 (9.2%) (0.7; 1.0) | *** |
Citrate | 0 | 0.6 (7.4%) (0.5; 0.8) | *** | 0.6 (6.9%) (0.5; 0.7) | *** | 0.8 (9.0%) (0.7; 0.9) | *** | 0.7 (8.4%) (0.6; 0.9) | *** |
5 min | 30 min (vs. 5 min) | 60 min (vs. 5 min) | 120 min (vs. 5 min) | 180 min (vs. 5 min) | |||||
---|---|---|---|---|---|---|---|---|---|
EDTA | 0 | 0.1 (1.2%) (−0.5; 0.6) | 0.1 (3.3%) (−0.4; 0.7) | 0.1 (3.1%) (−0.4; 0.7) | 0.2 (4.0%) (−0.4; 0.8) | ||||
Hirudin | 0 | 2.0 (51.2%) (1.4; 2.6) | *** | 2.3 (58.9%) (1.7; 2.9) | *** | 2.5 (54.8%) (1.9; 3.1) | *** | 2.7 (60.2%) (2.1; 3.1) | *** |
Citrate | 0 | 0.5 (19.1%) (−0.05; 1.1) | 0.7 (18.5%) (0.1; 1.3) | * | 0.9 (23.4%) (0.3; 1.5) | ** | 0.9 (22.4%) (0.3; 1.5) | ** |
5 min (vs. 30 min) | 30 min | 60 min (vs. 30 min) | 120 min (vs. 30 min) | 180 min (vs. 30 min) | ||||||
---|---|---|---|---|---|---|---|---|---|---|
ADP | Citrate + NaCl | −9 (−19.9%) (−15; −3) | ** | 0 | 1 (3.0%) (−5; 7) | −0.2 (0.1%) (−6; 6) | −9 (−17.1%) (−15; −3) | ** | ||
Citrate + CaCl2 | −24 (−35.5%) (−30; −18) | *** | 0 | 1 (2.6%) (−5; 7) | 4 (7.5%) (−2; 10) | −2 (1.0%) (−8; 4) | ||||
Hirudin | −7 (−10.0%) (−14; −1) | * | 0 | 4 (7.0%) (−2; 10) | 7 (11.6%) (1; 13) | * | 6 (10.0%) (−0.01; 12) | |||
TRAP | Citrate + NaCl | 1 (1.3%) (−6; 8) | 0 | −10 (−9.2%) (−17; −3) | ** | −13 (−11.8%) (−20; −6) | *** | −18 (−17.2%) (−25; −11) | *** | |
Citrate + CaCl2 | −36 (−29.5%) (−44; −29) | *** | 0 | −7 (−5.8%) (−14; 0.3) | −6 (−4.9%) (−13; 1) | −15 (−12.1%) (−22; −8) | *** | |||
Hirudin | −11 (−8.1%) (−18; −4) | ** | 0 | −2 (−1.2%) (−9; 5) | −10 (−6.8%) (−17; −3) | ** | −14 (−9.2%) (−21; −6) | *** |
5 min | 30 min | 60 min | 120 min | 180 min | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|
ADP | Citrate + CaCl2 (vs. Hirudin) | −20 (−30.3%) (−26; −14) | *** | −4 (−3.0%) (−10; 2) | −7 (−6.3%) (−13; −1) | * | −6 (−6.7%) (−12; −0.4) | * | −11 (−8.3%) (−17; −5) | *** | |
Citrate + CaCl2 (vs. + NaCl) | 3 (12.0%) (−3; 9) | 19 (41.5%) (13; 25) | *** | 19 (41.9%) (13; 25) | *** | 23 (52.1%) (17; 29) | *** | 26 (76.7%) (20; 32) | *** | ||
Citrate + NaCl (vs. Hirudin) | −24 (35.5%) (−30; −18) | *** | −22 (−29.2%) (−28; −16) | *** | −26 (−32.3%) (−32; −20) | *** | −29 (−36.0%) (−36; −23) | *** | −37 (−44.6%) (−43; −31) | *** | |
TRAP | Citrate + CaCl2 (vs. Hirudin) | −40 (−30.1%) (−47; −33) | *** | −14 (−9.9%) (−21; −7) | *** | −19 (−14.0%) (−26; −12) | *** | −11 (−8.0%) (−18; −3) | ** | −15 (−12.3%) (−23; −8) | *** |
Citrate + CaCl2 (vs. + NaCl) | −20 (17.5%) (−27; −13) | *** | 17 (18.5%) (10; 25) | *** | 21 (23.4%) (14; 28) | *** | 24 (27.9%) (17; 31) | *** | 21 (27.0%) (14; 28) | *** | |
Citrate + NaCl (vs. Hirudin) | −20 (−14.0%) (−27; −13) | *** | −32 (−23.4%) (−39; −25) | *** | −40 (−29.9%) (−47; −33) | *** | −35 (−27.5%) (−42; −28) | *** | −37 (−29.6%) (−44; −29) | *** |
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Hardy, M.; Lessire, S.; Kasikci, S.; Baudar, J.; Guldenpfennig, M.; Collard, A.; Dogné, J.-M.; Chatelain, B.; Jacqmin, H.; Lecompte, T.; et al. Effects of Time-Interval since Blood Draw and of Anticoagulation on Platelet Testing (Count, Indices and Impedance Aggregometry): A Systematic Study with Blood from Healthy Volunteers. J. Clin. Med. 2020, 9, 2515. https://doi.org/10.3390/jcm9082515
Hardy M, Lessire S, Kasikci S, Baudar J, Guldenpfennig M, Collard A, Dogné J-M, Chatelain B, Jacqmin H, Lecompte T, et al. Effects of Time-Interval since Blood Draw and of Anticoagulation on Platelet Testing (Count, Indices and Impedance Aggregometry): A Systematic Study with Blood from Healthy Volunteers. Journal of Clinical Medicine. 2020; 9(8):2515. https://doi.org/10.3390/jcm9082515
Chicago/Turabian StyleHardy, Michael, Sarah Lessire, Sultan Kasikci, Justine Baudar, Maité Guldenpfennig, Adrien Collard, Jean-Michel Dogné, Bernard Chatelain, Hugues Jacqmin, Thomas Lecompte, and et al. 2020. "Effects of Time-Interval since Blood Draw and of Anticoagulation on Platelet Testing (Count, Indices and Impedance Aggregometry): A Systematic Study with Blood from Healthy Volunteers" Journal of Clinical Medicine 9, no. 8: 2515. https://doi.org/10.3390/jcm9082515