Multispectral Optoacoustic Tomography: Intra- and Interobserver Variability Using a Clinical Hybrid Approach
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design and Subjects
2.2. Technical Aspects of MSOT Imaging Device
2.3. MSOT Image Acquisition
2.4. Data Analysis and Statistics
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Right Forearm | Left Forearm | Transversal Measurement | Longitudinal Measurement | |||||||
---|---|---|---|---|---|---|---|---|---|---|
Muscle | Mean (n = 12) | SD | Mean (n = 12) | SD | p-Value | Mean (n = 12) | SD | Mean (n = 12) | SD | p-Value |
Hb | 40.64 | 5.08 | 40.43 | 5.54 | 0.92 | 42.20 | 4.49 | 38.88 | 5.56 | 0.12 |
HbO2 | 35.94 | 6.64 | 33.01 | 7.81 | 0.33 | 37.52 | 6.94 | 32.64 | 7.99 | 0.12 |
HbT | 75.54 | 12.16 | 75.70 | 13.73 | 0.98 | 79.72 | 11.16 | 71.52 | 13.35 | 0.12 |
sO2 | 45.37 | 2.93 | 45.63 | 4.17 | 0.86 | 46.73 | 2.85 | 44.26 | 3.86 | 0.09 |
Subcutaneous Tissue | Mean (n = 12) | SD | Mean (n = 12) | SD | p-Value | Mean (n = 12) | SD | Mean (n = 12) | SD | p-Value |
Hb | 26.51 | 4.56 | 25.37 | 3.01 | 0.48 | 25.32 | 1.73 | 26.52 | 5.09 | 0.45 |
HbO2 | 29.01 | 8.44 | 30.21 | 7.18 | 0.71 | 29.73 | 3.20 | 29.49 | 10.64 | 0.94 |
HbT | 55.52 | 5.52 | 55.58 | 9.11 | 0.98 | 55.05 | 4.31 | 56.05 | 9.72 | 0.75 |
sO2 | 51.12 | 11.05 | 53.54 | 6.88 | 0.53 | 54.50 | 2.59 | 50.16 | 12.50 | 0.25 |
Proband 1 | Proband 2 | Proband 3 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
First Measurement | Second Measurement | First Measurement | Second Measurement | First Measurement | Second Measurement | |||||||
Muscle | Mean (n = 4) | SD | Mean (n = 4) | SD | Mean (n = 4) | SD | Mean (n = 4) | SD | Mean (n = 4) | SD | Mean (n = 4) | SD |
Hb | 40.54 | 3.8 | 40.64 | 3.61 | 40.72 | 2.60 | 31.92 | 4.14 | 44.61 | 1.48 | 44.81 | 2.64 |
HbO2 | 36.55 | 5.17 | 38.33 | 8.41 | 33.74 | 3.37 | 22.16 | 3.97 | 38.41 | 2.50 | 41.29 | 3.06 |
HbT | 77.09 | 8.95 | 78.97 | 12.01 | 74.46 | 5.73 | 54.07 | 7.97 | 83.02 | 3.71 | 86.09 | 5.56 |
sO2 | 46.65 | 1.88 | 47.86 | 4.26 | 44.40 | 2.05 | 40.16 | 2.81 | 46.10 | 1.19 | 47.80 | 0.80 |
Subcutaneous Tissue | Mean (n = 4) | SD | Mean (n = 4) | SD | Mean (n = 4) | SD | Mean (n = 4) | SD | Mean (n = 4) | SD | Mean (n = 4) | SD |
Hb | 28.85 | 5.65 | 27.59 | 3.93 | 27.08 | 3.20 | 24.61 | 2.12 | 22.32 | 1.04 | 25.18 | 0.65 |
HbO2 | 29.06 | 8.44 | 28.50 | 9.89 | 37.27 | 5.37 | 25.49 | 9.08 | 29.27 | 2.40 | 28.07 | 2.21 |
HbT | 57.91 | 3.39 | 56.09 | 6.30 | 64.35 | 5.51 | 50.10 | 10.48 | 51.59 | 2.74 | 53.25 | 2.80 |
Muscle | Subcutaneous Tissue | ||||||
---|---|---|---|---|---|---|---|
Hb | |||||||
Examiner 1 | Examiner 2 | Examiner 3 | Examiner 1 | Examiner 2 | Examiner 3 | ||
Proband 1 | 38.55 | 41.61 | 48.22 | Proband 1 | 23.99 | 24.26 | 25.64 |
Proband 2 | 35.49 | 35.94 | 38.07 | Proband 2 | 25.68 | 28.70 | 26.36 |
Proband 3 | 36.73 | 35.84 | 32.54 | Proband 3 | 18.21 | 19.52 | 14.83 |
Proband 4 | 40.52 | 37.66 | 39.56 | Proband 4 | 23.06 | 21.60 | 22.91 |
ICC | 0.50 | ICC | 0.86 | ||||
HbO2 | |||||||
Examiner 1 | Examiner 2 | Examiner 3 | Examiner 1 | Examiner 2 | Examiner 3 | ||
Proband 1 | 34.28 | 41.15 | 46.47 | Proband 1 | 27.15 | 30.21 | 28.95 |
Proband 2 | 26.17 | 32.26 | 28.31 | Proband 2 | 29.56 | 29.22 | 30.01 |
Proband 3 | 35.48 | 37.87 | 31.26 | Proband 3 | 25.18 | 27.47 | 22.10 |
Proband 4 | 38.28 | 34.08 | 34.95 | Proband 4 | 29.68 | 29.15 | 28.46 |
ICC | 0.52 | ICC | 0.60 | ||||
HbT | |||||||
Examiner 1 | Examiner 2 | Examiner 3 | Examiner 1 | Examiner 2 | Examiner 3 | ||
Proband 1 | 72.82 | 82.76 | 94.69 | Proband 1 | 51.14 | 54.48 | 54.59 |
Proband 2 | 61.66 | 68.19 | 66.39 | Proband 2 | 55.24 | 57.93 | 56.38 |
Proband 3 | 72.21 | 73.71 | 63.80 | Proband 3 | 43.38 | 47.00 | 36.93 |
Proband 4 | 78.80 | 71.74 | 74.51 | Proband 4 | 52.74 | 50.75 | 51.37 |
ICC | 0.49 | ICC | 0.81 | ||||
sO2 | |||||||
Examiner 1 | Examiner 2 | Examiner 3 | Examiner 1 | Examiner 2 | Examiner 3 | ||
Proband 1 | 47.06 | 49.78 | 49.04 | Proband 1 | 53.67 | 56.22 | 53.73 |
Proband 2 | 42.32 | 46.91 | 42.56 | Proband 2 | 52.97 | 50.27 | 53.41 |
Proband 3 | 49.02 | 51.47 | 49.15 | Proband 3 | 59.32 | 59.29 | 60.68 |
Proband 4 | 48.05 | 47.60 | 46.66 | Proband 4 | 56.25 | 58.65 | 53.06 |
ICC | 0.69 | ICC | 0.72 |
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Helfen, A.; Masthoff, M.; Claussen, J.; Gerwing, M.; Heindel, W.; Ntziachristos, V.; Eisenblätter, M.; Köhler, M.; Wildgruber, M. Multispectral Optoacoustic Tomography: Intra- and Interobserver Variability Using a Clinical Hybrid Approach. J. Clin. Med. 2019, 8, 63. https://doi.org/10.3390/jcm8010063
Helfen A, Masthoff M, Claussen J, Gerwing M, Heindel W, Ntziachristos V, Eisenblätter M, Köhler M, Wildgruber M. Multispectral Optoacoustic Tomography: Intra- and Interobserver Variability Using a Clinical Hybrid Approach. Journal of Clinical Medicine. 2019; 8(1):63. https://doi.org/10.3390/jcm8010063
Chicago/Turabian StyleHelfen, Anne, Max Masthoff, Jing Claussen, Mirjam Gerwing, Walter Heindel, Vasilis Ntziachristos, Michel Eisenblätter, Michael Köhler, and Moritz Wildgruber. 2019. "Multispectral Optoacoustic Tomography: Intra- and Interobserver Variability Using a Clinical Hybrid Approach" Journal of Clinical Medicine 8, no. 1: 63. https://doi.org/10.3390/jcm8010063
APA StyleHelfen, A., Masthoff, M., Claussen, J., Gerwing, M., Heindel, W., Ntziachristos, V., Eisenblätter, M., Köhler, M., & Wildgruber, M. (2019). Multispectral Optoacoustic Tomography: Intra- and Interobserver Variability Using a Clinical Hybrid Approach. Journal of Clinical Medicine, 8(1), 63. https://doi.org/10.3390/jcm8010063