Multiscale Dynamics of Lipid Vesicles in Polymeric Microenvironment
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Liposome Solutions
2.2. Preparation of Liposome Solutions Containing Oil Red O
2.3. Preparation of Liposome–Polymer Mixtures
2.4. Dynamic Light Scattering (DLS)
2.5. Differential Scanning Calorimetry (DSC)
2.6. Rheology
2.7. Small-Angle X-ray Scattering (SAXS)
2.8. Cryo-TEM
2.9. UV-Vis Spectroscopy
2.10. Time-Resolved Photoluminescence
3. Results and Discussion
3.1. Structure
3.2. Dynamics
3.2.1. Bulk Diffusion of Vesicles
3.2.2. Macroscopic Viscosity
3.2.3. Microscopic Viscosity in Bilayers
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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HD (nm) (35 °C) | PDI | Tc (°C) | Specific Enthalpy (J/g) | DT (m2/s) (35 °C) (x 10−12) | DT (m2/s) (15 °C) (x 10−12) | |
---|---|---|---|---|---|---|
Liposome only | 119.9 ± 1.11 | 0.075 ± 0.010 | 22.84 ± 0.11 | 0.335 ± 0.013 | 5.21 ± 0.018 | 3.73 ± 0.013 |
In PEG 1.5 kDa | 121.4 ± 0.20 | 0.074 ± 0.009 | 23.55 ± 0.19 | 0.242 ± 0.015 | 5.12 ± 0.020 | 3.61 ± 0.015 |
In PEG 20 kDa | 129.6 ± 1.10 | 0.077 ± 0.014 | 23.39 ± 0.02 | 0.241 ± 0.017 | 5.03 ± 0.023 | 3.58 ± 0.016 |
In PEG 100 kDa | 135.7 ± 2.13 | 0.113 ± 0.022 | 23.95 ± 0.32 | 0.234 ± 0.020 | 5.02 ± 0.028 | 3.21 ± 0.020 |
In PEG 400 kDa | 157.7 ± 3.28 | 0.150 ± 0.025 | 22.58 ± 0.07 | 0.256 ± 0.012 | 4.40 ± 0.019 | 2.72 ± 0.014 |
τavg (ns) 35 °C | Microviscosity (cP) 35 °C | Bulk Viscosity (cP) 35 °C | τavg (ns) 15 °C | Microviscosity (cP) 15 °C | Bulk Viscosity (cP) 15 °C | |
---|---|---|---|---|---|---|
100 nm liposomes | 24.01 ± 0.032 | 28.75 ± 0.85 | 1.414 | 28.91 ± 0.038 | 142.82 ± 13.54 | 1.546 |
in PEG 1.5 kDa | 20.61 ± 0.013 | 1.02 ± 0.01 | 1.156 | 27.38 ± 0.033 | 3.36 ± 0.29 | 1.827 |
in PEG 20 kDa | 21.43 ± 0.022 | 2.39 ± 0.05 | 3.406 | 27.59 ± 0.036 | 5.69 ± 0.53 | 4.163 |
in PEG 100 kDa | 22.72 ± 0.019 | 8.59 ± 0.15 | 6.874 | 28.42 ± 0.029 | 43.93 ± 3.2 | 8.247 |
in PEG 400 kDa | 23.91 ± 0.028 | 26.24 ± 0.68 | 23.94 | 28.74 ± 0.031 | 95.09 ± 7.34 | 53.35 |
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Karaz, S.; Han, M.; Akay, G.; Onal, A.; Nizamoglu, S.; Kizilel, S.; Senses, E. Multiscale Dynamics of Lipid Vesicles in Polymeric Microenvironment. Membranes 2022, 12, 640. https://doi.org/10.3390/membranes12070640
Karaz S, Han M, Akay G, Onal A, Nizamoglu S, Kizilel S, Senses E. Multiscale Dynamics of Lipid Vesicles in Polymeric Microenvironment. Membranes. 2022; 12(7):640. https://doi.org/10.3390/membranes12070640
Chicago/Turabian StyleKaraz, Selcan, Mertcan Han, Gizem Akay, Asim Onal, Sedat Nizamoglu, Seda Kizilel, and Erkan Senses. 2022. "Multiscale Dynamics of Lipid Vesicles in Polymeric Microenvironment" Membranes 12, no. 7: 640. https://doi.org/10.3390/membranes12070640